Amplification primer for detecting mink derived ingredients
A technology for amplification primers and water detection, which is applied in the field of amplification primers for detection of mink-derived components, and achieves the effect of species specificity and high sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Example 1 Establishment of the PCR detection kit and detection method of mink-derived component DNA
[0028] 1. Design of PCR detection primers for mink-derived DNA
[0029] From GenBank, 39 related mitochondrial sequences of carnivorous mustelids were retrieved, including mustelids, mink, otter, etc. This study refers to sable, mink bear, Japanese mink, blue fox, silver fox, raccoon dog, etc. published in GenBank For the mitochondrial genome sequence of animals, the sequences were compared by blast sequence. According to the conserved sequence of the species of sable and the analysis of specific sites, the primer design software primer5. Specific fragments of mink DNA are added, and no other animal DNA restriction fragments can be amplified.
[0030] 1. In the early stage of the experiment, based on the above principles, six pairs of primers were designed, named mink01 to mink06 respectively, and the designed primers were compared with the mitochondrial sequences of s...
Embodiment 2
[0064] Example 2 PCR detection method of mink DNA Specific detection experiment of mink-derived component DNA in meat and blood samples
[0065] In view of the phenomenon that mink meat is used as mutton rolls in the market, this test detects mink-derived components from the perspective of specific primer amplification fragments, in order to provide a detection method for detecting whether mink meat is adulterated in mutton rolls. It provides a molecular detection method for the quality inspection department. Mutton, beef, and chicken were purchased in the market and the genome was extracted for later use. DNA extraction from muscle tissue was done using the aforementioned kit. The detection kit and detection method containing specific primers of Example 1 were used to detect mink DNA samples. At the same time, the DNA samples of other fur animals such as blue fox and raccoon dog were detected to evaluate the specificity of this method.
[0066] The test results show that: ...
Embodiment 3
[0067] Example 3 The specificity experiment of the PCR detection method of mink-derived components to DNA in fur
[0068] Fur products are high-end consumer goods. Driven by profit, many people shoddy and even used fake artificial fur as mink fur, or used rabbit fur, dog skin, etc. as mink fur. Through further experimental means can be effectively identified and distinguished. The design of this experiment is as follows. Several kinds of animal fur were selected, and the DNA was extracted by the method of extracting genomic DNA from the fur. The genomic DNA of mink fur, fox fur, raccoon fur, dog skin and rabbit skin was respectively obtained, and the kit in Example 1 was used. And method for specific band test, the experimental results show that the above results can prove that the primers are only specific to mink.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com