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Test paper for detecting urine creatinine and preparation method for test paper

A technology for detecting test paper and urine creatinine, which is applied in the direction of analyzing materials through chemical reactions and material analysis through observing the influence of chemical indicators, etc. It can solve the problems of measurement interference, easy degradation of fibers, and unfavorable long-term stable storage of test papers. and other problems, to achieve the effect of easy machine readability, simple preparation method, and overcoming the difficulty of long-term stable storage

Inactive Publication Date: 2012-09-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is similar to the determination of creatinine by the picric acid method, but there is a problem that false creatinine interferes with the determination, and the complexation needs to be carried out under strong alkaline conditions. The presence of strong alkaline components makes the test paper easy to absorb water and carbon dioxide, and the fiber is easy to degrade. Very unfavorable for long-term stable storage of test paper

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Liquid A (1000mL) contains:

[0017] 2mol / L Tris buffer, pH: 6.0,

[0018] Copper sulfate 02g,

[0019] Sodium citrate 1.0g,

[0020] Orange G100mg,

[0021] Dilute pure water to 1000mL.

[0022] Liquid B (1000mL) contains:

[0023] Polyvinylpyrrolidone 10g,

[0024] 3,3',5,5'-Tetramethylbenzidine 0.5g,

[0025] Chloroform to 1000mL.

[0026] Prepare liquid A and liquid B separately. Chromatographic, qualitative or quantitative filter paper (weight greater than 240g / m 2 ) fully immersed in liquid A, take it out and dry it in an oven at 70°C for 15min. Then immerse the filter paper in liquid B, take it out and dry it in an oven at 70°C for 5 minutes.

Embodiment 2

[0028] Liquid A (1000mL) contains:

[0029] 2mol / L Tris buffer, pH: 7.0,

[0030] Copper sulfate 1.0g,

[0031] Sodium citrate 2.5g,

[0032] Orange G150mg,

[0033] Dilute pure water to 1000mL.

[0034] Liquid B (1000mL) contains:

[0035] Polyvinylpyrrolidone 20g,

[0036] 3,3',5,5'-Tetramethylbenzidine 2.5g,

[0037] Chloroform to 1000mL.

[0038] Prepare liquid A and liquid B separately. Chromatographic, qualitative or quantitative filter paper (weight greater than 240g / m 2 ) fully immersed in liquid A, take it out and dry it in an oven at 85°C for 20min. Then immerse the filter paper in B solution, take it out and put it in an oven to dry at 85°C for 10min.

Embodiment 3

[0040] Liquid A (1000mL) contains:

[0041] 2mol / L Tris buffer, pH: 8.0,

[0042] Copper sulfate 2.0g,

[0043] Potassium citrate 5.0g,

[0044] Orange G200mg.

[0045] Dilute pure water to 1000mL.

[0046] Liquid B (1000mL) contains:

[0047] Polyvinylpyrrolidone 15g,

[0048] 3,3',5,5'-Tetramethylbenzidine 5.0g,

[0049] Chloroform to 1000mL.

[0050] Prepare liquid A and liquid B separately. Chromatographic, qualitative or quantitative filter paper (weight greater than 240g / m 2 ) fully immersed in liquid A, take it out and dry it in an oven at 100°C for 30min. Then soak the filter paper in solution B, take it out, and put it in an oven to dry at 100°C for 15 minutes. This test strip is used for urine creatinine test and has the highest color clarity.

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Abstract

The invention discloses a test paper for detecting urine creatinine and a preparation method for the test paper. The test paper belongs to in-vitro diagnosis test paper. The preparation method comprises the following steps: fully soaking a color sheet in a solution A, taking out the color sheet, drying at the temperature of between 70 and 100DEG C for 15 to 30 minutes, soaking the dried color sheet in a solution B, and drying at the temperature of between 70 and 100DEG C for 5 to 15 minutes, wherein each 1,000mL of solution A contains 2mol / L Tris buffer (with the pH of 6.0-8.0), 0.2 to 2.0g of copper sulfate, 1.5 to 5.0g of sodium citrate or potassium citrate, 100 to 200mg of orange G, and the rest of pure water; and each 1,000mL of solution B contains 10 to 20g of polyvinylpyrrolidone, 0.5 to 5.0g of reducing dyestuff and the rest of chloroform. The test paper is easy to prepare, stable in performance and easy to store, has obvious color gradation corresponding to different urine creatinine content, and is easily used by laboratory medicine of hospitals and nonprofessional inspectors.

Description

technical field [0001] The invention relates to a urine creatinine detection test paper and a preparation method thereof, belonging to in vitro diagnostic reagents. technical background [0002] 90% of the creatine in the human body exists in the muscles, and most of it exists in the form of phosphocreatine. Creatine phosphate is dephosphorylated to produce creatinine, which is excreted in urine as creatinine. Urinary creatinine mainly comes from the blood and is excreted with urine after being filtered by the glomerulus. A urine creatinine test measures the amount of creatinine in the blood that is filtered out by the kidneys. Normal range of urine creatinine value: 8.4 ~ 13.25mmol / 24h urine or 40 ~ 130mg / dl urine. The daily excretion of urinary creatinine in normal people is quite stable, basically not affected by food protein content and urine output. When the creatinine content in the urine is significantly increased or decreased, it reflects that the renal function ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 陈国松张琳昀黄招霞魏寒桥张之翼
Owner NANJING UNIV OF TECH
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