Method for preparing lovastatin
A technology of lovastatin and seeds, applied in the field of preparation of lovastatin, capable of solving the problems of high production cost and low yield
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Embodiment 1
[0018] Example 1 Effect of Adding Phenylalanine on Lovastatin Production
[0019] 1.1 The effect of the addition amount of phenylalanine on the biosynthesis of lovastatin
[0020] Prepare the fermentation medium of lovastatin, add the weight ratio phenylalanine shown in Table 1 respectively to the lovastatin fermentation medium, meanwhile, use the fermentation medium without adding phenylalanine as a control, insert soil After Aspergillus terreus was fermented for 216 hours, samples were taken to determine the content of lovastatin. The results are shown in Table 1.
[0021] Table 1, the impact of adding phenylalanine on the output of lovastatin
[0022] Addition of phenylalanine (%)
[0023] As can be seen from the results in Table 1, adding phenylalanine to the fermentation medium can significantly improve the biosynthesis of lovastatin, and the preferred addition range is 0.01-0.03%.
[0024] 1.2 The effect of the addition time of phenylalanine on the yield of...
Embodiment 2
[0029] Example 2 The effect of adding tyrosine on the biosynthesis of lovastatin
[0030] 2.1 The effect of the addition amount of tyrosine on the biosynthesis of lovastatin
[0031] To prepare the fermentation medium of lovastatin, add 0.005%, 0.01%, 0.03%, and 0.05% tyrosine by weight respectively to the lovastatin fermentation medium; meanwhile, use the fermentation medium without adding tyrosine As a control, Aspergillus terreus was inoculated and fermented at 26±1° C. for 216 hours, then samples were taken to determine the content of lovastatin. The results are shown in Table 3.
[0032] Table 3, the impact of adding tyrosine on the yield of lovastatin
[0033] Tyrosine addition (%)
0
0.005
0.01
0.03
0.05
Relative content
100
108
116
115
109
[0034] It can be seen from the results in Table 3 that adding tyrosine to the fermentation medium can obviously improve the biosynthesis of lova...
Embodiment 3
[0041] Example 3 Effects of Simultaneous Addition of Phenylalanine and Tyrosine on the Biosynthesis of Lovastatin
[0042]3.1 The effect of simultaneous addition of phenylalanine and tyrosine on the biosynthesis of lovastatin
[0043] Prepare the fermentation medium of lovastatin, add the tyrosine and phenylalanine that are 0.005%, 0.01%, 0.03%, 0.05% by weight respectively in the lovastatin fermentation medium at the same time, meanwhile, without adding tyrosine As a control, the fermentation medium of Aspergillus terreus was inserted into Aspergillus terreus and fermented at 26±1° C. for 216 hours, and then samples were taken to determine the content of lovastatin. The results are shown in Table 5.
[0044] Table 5, adding phenylalanine and tyrosine simultaneously to the influence of lovastatin output
[0045]
[0046] It can be seen from the results in Table 5 that adding phenylalanine and tyrosine to the fermentation medium can significantly improve the biosynthesis...
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