Goat SH2B1 gene single nucleotide polymorphism loci and detection method thereof
A single nucleotide polymorphism, SH2B1 technology, applied in the field of molecular genetics, to achieve the effect of low cost and high accuracy
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[0031] In order to facilitate the understanding of the technical solution of the present invention, the present invention will be further described below in conjunction with specific examples.
[0032] The present invention designs primers according to the conserved sequence of the SH2B1 gene, uses genomic DNAs of four goat populations as templates, performs PCR amplification, mixes PCR products, purifies them, and sequences them. Then, carry out sequencing map analysis and sequence comparison to screen out SNP sites; secondly, carry out PCR-SSCP detection of polymorphic sites in the tested population; finally, carry out statistical analysis of population genetics according to the genotypes detected in the population The association analysis with body size traits screened out molecular markers closely related to goat body size traits. The present invention will be described in detail below, which is an explanation of the present invention rather than a limitation.
[0033] 1....
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