Plant lateral branch number correlated transcription factor AtDOF 4.2, its encoding gene and application

A technology for encoding genes and plants, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve problems such as the reduction and loss of side branch development

Inactive Publication Date: 2014-03-05
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its deletion mutants lead to a reduction or loss of collateral development

Method used

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  • Plant lateral branch number correlated transcription factor AtDOF 4.2, its encoding gene and application
  • Plant lateral branch number correlated transcription factor AtDOF 4.2, its encoding gene and application
  • Plant lateral branch number correlated transcription factor AtDOF 4.2, its encoding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1, the cloning of Arabidopsis AtDOF4.2 gene

[0069] 1. Cloning of AtDOF4.2 gene

[0070]To clone the AtDOF4.2 gene, Arabidopsis thaliana cv Columbia-0, Col-0, hereinafter referred to as wild-type Arabidopsis thaliana, was purchased from Arabidopsis Center Arabidopsis Biological Resource Center) total RNA and mRNA were purified using a Promega kit (purchased from Promega) PolyAT tract mRNA isolation system IV to separate, and the isolated mRNA was quantified with an ultraviolet spectrophotometer. The cDNA obtained by reverse transcription was used as a template for PCR amplification, and the primer sequences were 5'-ATGAATAATTTGAATGTTTT-3' (sequence 3) and 5'-TTATGATTCATATTCAAAT-3' (sequence 4).

[0071] The PCR product was detected by 0.8% agarose gel electrophoresis, and the result showed that the size of the PCR amplification product was about 0.5 kb, which was consistent with the expected result. This fragment was recovered using an agarose gel recovery...

Embodiment 2

[0085] Example 2, AtDOF4.2 transgenic Arabidopsis and its functional research

[0086] 1. Identification of T-DNA mutants of AtDOF4.2 gene

[0087] T-DNA insertion mutant dof4.2 (CS813276) was purchased from ABRC (Arabidopsis Biological Resource Center).

[0088] Seeds of wild-type Arabidopsis and dof4.2 mutants were grown in petri dishes for 2 weeks and then transplanted (grown at 22°C with a daily light of 16h / 8h (light / dark)) into pots. After flowering, the whole plant was taken Extract RNA for RT-PCR identification.

[0089] Primer is AtDof4.2 5'-ATGAATAATTTGAATGTTTT-3' (SEQ ID NO: 3)

[0090] AtDof4.2 5'-TTATGATTCATATTCAAAT-3' (SEQ ID NO: 4)

[0091] Actin was used as an internal reference, and the primers were

[0092] Actin F 5'-GAAGATTAAGGTCGTTGCACCACCTG3'

[0093] Actin R 5'-ATTAACATTGCAAAAGAGTTTCAAGGT3'

[0094] The result is as figure 2 As shown, Col-0 is wild-type Arabidopsis, and dof4.2 is a mutant. It can be seen from the figure that AtD...

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Abstract

The invention discloses a plant lateral branch number correlated transcription factor AtDOF 4.2, its encoding gene and an application. A protein provided by the invention is one of the following proteins (a) and (b): (a) a protein composed of amino acid sequence as shown in Sequence 2 in a sequence table; and (b) a protein which is correlated with the plant lateral branch number and is derived from (a) after one or more amino acid residue substitution and / or deletion and / or addition of the amino acid sequence as shown in the Sequence 2 in the sequence table. It is proved through experiments that the cloned AtDOF 4.2 gene has a certain value of cultivating plant varieties with high lateral branch number, especially new species such as greening-related forest trees, flowers and lawn grass and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant side branch number-related transcription factor AtDOF4.2 and its coding gene and application. Background technique [0002] The development of plant lateral branches plays a very important role in plant morphogenesis, and is the main development process for plants to form the morphological structure of roots, stems and inflorescences. Branching of crops is one of the important agronomic traits that affect crop yield. Maize can increase yield by reducing the number of branches, and the number of tillers in rice will also directly determine its yield. The plant type of the aerial part of the plant is derived from the shoot apical meristem (SAM) during embryonic development and the axillary meristem (AM) during postembryonic development. SAM produces the main axis of the plant, while AM ​​differentiates to produce axillary buds and eventually develops to form side branches. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 陈受宜张劲松邹洪锋张万科林晴马彪
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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