Compositions and methods for detecting and treating implant loosening and osteolysis
A technology for osteolysis and implants, applied in the field of osteolysis and implant loosening, can solve problems such as difficult to detect bone changes in imaging process, high radiation exposure, expensive, etc.
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[0057] Materials and methods
[0058] Synthesis of Poly(HPMA-co-APMA)
[0059] N-(2-hydroxypropyl)methacrylamide (HPMA; 1g, 6.98mmol; (1973) Eur.Polymer J., 9:7-14), N-(3-aminopropyl)methacrylamide hydrochloride (APMA, 12.5mg, 0.07mmol, Polysciences, Inc., Warrington, PA ), azobisisobutyronitrile (AIBN, 6.74 mg, 41 μmol, Sigma-Aldrich, Milwaukee, WI), S,S'-bis(a,a'-dimethyl-a"-acetic acid) trithiocarbonic acid Ester (CTA, 6.26 mg, 23.6 μmol, purity > 97%) (Lai et al. (2002) Macromolecules 35: 6754-6) and N-methacryloylaminopropyl fluorescein thiourea (MA-FITC, 4 mg) ( Omelyanenko etc. (1998) J.ControlRelease 53:25-37) is dissolved in 8mL methanol, is placed in the ampoule, and with N 2 Purge for 5 minutes. The ampoules were flame sealed and kept at 40°C in the dark for 48 hours. The product was purified by LH-20 column (GE HealthCare, Piscataway, NJ) to remove unreacted low molecular weight compounds and then lyophilized. The final yield was 0.42 g. The amine content o...
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