Non-invasive extraction method of Haliotis discus hannai ino genome DNA

Abstract

The invention discloses a non-invasive extraction method of Haliotis discus hannai ino genome DNA, comprising the steps of using 75% of alcohol to sterilize and irritate pallium of Haliotis discus hannai ino; scraping to obtain a mixture of mucus and epidermal tissue fragments; treating the mixture, centrifugally separating the mixture to obtain precipitate; adding a cell lysate and protease K to the precipitate; after digesting and oscillating, cooling the mixture for a certain time; adding NH4Ac to the sample; centrifugally separating to collect supernate; adding isopropanol to the supernate; after oscillating and mixing uniformly, and then centrifugally separating to obtain precipitate; using ethanol to wash the precipitate; drying the precipitate at room temperature for 5 minutes to obtain the Haliotis discus hannai ino genome DNA. The non-invasive extraction method of the Haliotis discus hannai ino genome DNA is simple, efficient and convenient, does not damage the Haliotis discus hannai ino and does not influence survival ability and production performance.

Description

technical field

[0001] The invention relates to a method for extracting genomic DNA, in particular to a method for extracting genomic DNA without damage. Background technique

[0002] Haliotisdiscus hannai belongs to the mollusca phylum Gastropoda, Probranchia, and Primitive Gastropoda. It is the only species of Baalaceae shellfish distributed in northern my country, and it is also an important object of mariculture in Liaoning and Shandong coasts. Using modern molecular biology techniques, the population genetic structure analysis and genetic improvement of Pan abalone must be extracted from Genomic DNA. The traditional animal genome extraction methods mainly use muscle tissue or peripheral blood cells as DNA extraction objects. These methods will inevitably lead to wrinkled abalone in a state of stress, causing permanent damage or even death, making it impossible for the test object to continue to be used in production. At present, the Walk-back selection method based on ...