A cleft lip and palate susceptibility gene detection chip and detection method

A susceptibility gene and detection chip technology, which is applied in the field of gene chips, can solve the problems of heat-labile activity and reduction of enzymes, and achieve the effects of improving conditions, accurately detecting, and reducing cleft lip and palate

Inactive Publication Date: 2015-08-19
LIAONING RES INST OF FAMILY PLANNING
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

(5) Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folic acid metabolism, and the mutation of C677T encoding this enzyme will lead to heat intolerance and reduced activity of the enzyme
[0005]Based on the fact that the above-mentioned genes may be related to the occurrence of cleft lip and palate, there is no research involving the use of gene chip methods to detect cleft lip and palate susceptible population at home and abroad

Method used

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  • A cleft lip and palate susceptibility gene detection chip and detection method
  • A cleft lip and palate susceptibility gene detection chip and detection method
  • A cleft lip and palate susceptibility gene detection chip and detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0020] 1. Sample DNA extraction: Take 200ul sample and use blood DNA extraction kit (Tiangen Biochemical Technology) to extract the DNA of the sample, set aside.

[0021] 2. Selection of susceptibility loci, primer and probe sequence design

[0022] 1) According to the existing susceptibility genes related to cleft lip and palate, each susceptibility gene was classified and counted by meta-analysis method, the OR value was calculated, and the susceptibility loci related to the occurrence of cleft lip and palate in the Chinese population were counted. Taking the Taq I gene locus of TGFA as an example, the data of cases and controls in the literature of TGFATaq I locus of all Chinese people who studied cleft lip and palate were collected and calculated with review manager 4.2 software. The results are as follows figure 1 , the OR value of this site is 2.59, greater than 1, so this site is a risk factor for cleft lip and palate. OR values ​​and SNP polymorphic nucleic acid sequ...

Embodiment 2

[0082] Take the blood to be tested and perform the test according to the operation steps of the above-mentioned embodiments. For the scan results, see Figure 4 , the chip results are as follows.

[0083] Table 4

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Abstract

The invention relates to gene chip, and specifically relates to a cleft lip and palate susceptibility gene detection chip and a detection method employing the chip. The chip comprises a substrate and nucleic acid probes positioned on the substrate. The nucleic acid probes comprise nucleic acids with nucleotide sequences represented by SEQ ID No.1-34. The 3' sites of the probes are subjected to amino modification. The gene chip of the cleft lip and palate susceptibility gene is used for detecting the relationships between various genes and cleft lip and palate. According to the gene chip, probes of sites of all the genes and possible to be related with cleft lip and palate are integrated into one chip. A base complementary hybridization method is adopted, such that high-flux, raft, and accurate detections are realized. With the cleft lip and palate susceptibility gene detection chip, gene screening upon susceptible populations can be rapidly and conveniently carried out, high-risk populations can be detected within early pregnancy or pre-pregnancy periods, such that occurrences of cleft lip and palate can be prevented and reduced through means of reducing the contact with risk factors and improving conditions.

Description

technical field [0001] The invention relates to a gene chip, in particular to a cleft lip and palate susceptibility gene detection chip and a detection method. Background technique [0002] Nonsyndromic cleft with or without cleft palate (NSCL / P) is a relatively common congenital facial defect that does not belong to any syndrome and is not accompanied by other systemic organ abnormalities. One of the most common congenital malformations. The treatment of non-syndromic cleft lip and palate requires the intervention of various disciplines such as surgery, nutrition, dentistry, language, internal medicine, and behavior, which brings a significant economic burden to society and families. The incidence rate of cleft lip and palate of different races in different regions of the world is quite different, with an average of 1 / 700. The incidence rate in my country is relatively high, at 1 / 500. Although the exact etiology of cleft lip and palate is still inconclusive, genetic and e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C40B40/06
Inventor 韩维田卢永平刘强胥威李增建李建新
Owner LIAONING RES INST OF FAMILY PLANNING
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