Method for simultaneously determining content of three components for traditional Chinese medicine phyllanthus emblica through whole-time three-wavelength fusion
A technology of Emilia Radix and full time period is applied in the field of simultaneous determination of the content of three components by three-wavelength fusion in the whole period of traditional Chinese medicine Emmanuel Radix. Ensure the standardization of the process and the effect of simple operation
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[0030] The method for simultaneously determining the content of three components by three-wavelength fusion of the traditional Chinese medicine Emlical emblica in a full period of time comprises the following steps:
[0031] 1. Preparation of the test solution:
[0032] Take 3 g of emblica medicinal material (passed through a 16-mesh sieve), accurately weigh it, put it in a round-bottomed flask, add 33 mL of 70% ethanol accurately, heat to reflux for 1.5 hours, extract three times, recover the combined filtrate, add water to make the solution final The concentration is 0.15g / ml, and 17mL of the filtrate is accurately absorbed, added to the polyamide resin column, washed with 90mL of distilled water, and then eluted with 105mL of 50% ethanol. The ethanol eluate is collected, evaporated to dryness, and the residue is dissolved by adding methanol. Transfer to a 10mL measuring bottle, add methanol to make up to the mark, shake well, filter, and take the filtrate to obtain the fina...
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