Method for detecting liver-enhancing medicine

A detection method and drug technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as greater difficulty and complex interactions of traditional Chinese medicine ingredients, and achieve the effects of easy to master, perfect quality control methods, and good reproducibility.

Active Publication Date: 2014-07-02
SHIJIAZHUANG DONGFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the detection methods are limited to the determination of one or two index components in the large compound formula of Qianggan Capsules, but how to carry out macroscopic quality control methods for the quality of Qianggan Capsule preparations from a macroscopic point of view has not been reported
At the same time, since the raw materials of Qianggan Capsules contain 16 traditional Chinese medicines and there are complex interactions among the components of the traditional Chinese medicines, it is difficult to establish a comprehensive and effective detection method for active ingredients that can reflect the internal quality and curative effect of Qianggan Capsules. Therefore, currently There is a need in the art for a detection method capable of comprehensively characterizing the drug Qianggan Capsules

Method used

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  • Method for detecting liver-enhancing medicine
  • Method for detecting liver-enhancing medicine
  • Method for detecting liver-enhancing medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1 adopts HPLC to establish the multi-component assay method of Qianggan capsule contents

[0073] Preparation of mixed reference solution: Accurately weigh gentiopicroside 7.55mg, paeoniflorin 3.76mg, and salvianolic acid B 5.66mg respectively into 25ml measuring bottles, dissolve and dilute with methanol to the mark, shake well, and use as stock solutions. Precisely measure 2ml of gentiopicroside, paeoniflorin stock solution, and salvianolic acid B stock solution into a 10ml measuring bottle, dilute to the mark with methanol, and shake well to obtain a mixed reference solution.

[0074] Preparation of the test solution: Weigh about 0.3g of the content of Qianggan Capsules, accurately weigh, place in a conical flask or a 25ml volumetric flask, accurately add 25mL of 70% volume fraction methanol aqueous solution, weigh, sonicate or heat to reflux After 30 minutes, put it to room temperature, make up the weight with 70% methanol aqueous solution, shake well, ...

Embodiment 2

[0083] The investigation of need testing solution preparation method in the multi-component detection method of embodiment 2

[0084] Investigation on the preparation method of the test solution

[0085] 1. Investigation of extraction solvent

[0086] Take about 0.5g of the sample, weigh it accurately, add methanol, 70% methanol water solution, 75% ethanol water solution, and 50% ethanol water solution each 25ml as the extraction solvent, seal it tightly, weigh it, ultrasonicate for 30 minutes, take it out, and place it until At room temperature, weigh, make up the weight with the corresponding solvent, shake well, filter through a 0.45 μm microporous membrane, and take the subsequent filtrate for determination according to the determined chromatographic conditions. The test data are shown in Table 3 below.

[0087] The selection test data of table 3 extraction solvent

[0088] solvent for extraction

Gentiopicroside (area / g)

Paeoniflorin (area / g)

Salv...

Embodiment 3

[0105] Methodological investigation of embodiment 3 multi-component detection method

[0106] 1) Precision test

[0107] Adopt the operation and condition identical with embodiment 1, get about 0.3g of sample, accurately weigh, process by need testing solution preparation method, precision draw need testing solution 10 μ L, inject high-performance liquid chromatograph, in above-mentioned chromatographic condition The RSD values ​​were 0.55%, 0.69% and 1.48% for 6 consecutive injections. The precision is good.

[0108] 2) Stability inspection

[0109] Using the same operation and conditions as in Example 1, take about 0.3 g of the sample, prepare and extract according to the test solution, inject samples at 0, 1, 3, 6, 9, 12, and 24 hours respectively, and measure under the above-mentioned chromatographic conditions . As a result, the RSD of gentiopicroside was 0.77%, the RSD of paeoniflorin was 1.09%, and the RSD of salvianolic acid B was 0.75%. It shows that the test s...

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Abstract

The invention provides a method for detecting a liver-enhancing medicine. The liver-enhancing medicine is composed of oriental wormwood, isatis root, angelica, white paeony root, danshen root, Radix curcumae, Astragalus mongholicus, Codonopsis pilosula, Rhizoma alismatis, sealwort, rehmannia, yam, hawthorn, large-leaved gentian, liquorice and medicated leaven. The detecting method comprises the step of detecting paeoniflorin, gentiamarin and salvianolic acid B in the liver-enhancing medicine by using a high efficiency liquid chromatography method, wherein conditions are as follows: a chromatographic column takes octadecylsilane chemically bonded silica as a filling material; mobile phases comprise a mobile phase A which is acetonitrile and a mobile phase B which is an acidic water solution, and the mobile phases are subjected to gradient elution; the flow velocity is 1.0mL / min; the column temperature is 30 DEG C; the detection wavelength is 210nm to 400nm; and the number of theoretical plates is calculated according to the paeoniflorin peak and should not be less than 6000. According to the detecting method provided by the invention, the content of active ingredients of the paeoniflorin, the gentiamarin and the salvianolic acid B in the liver-enhancing medicine can be detected at the same time, so that the active ingredients of the liver-enhancing medicine can be comprehensively represented, and the method has the characteristics of high precision, high stability and high repeatability.

Description

technical field [0001] The invention belongs to the field of drug analysis and detection, and relates to a detection method for a liver-enhancing drug, in particular to a detection method for simultaneously detecting multiple components of the liver-enhancing drug by using high performance liquid chromatography (HPLC). Background technique [0002] Compound Chinese medicine is the main form of TCM clinical medication, and it is the characteristic and essence of TCM. Traditional Chinese medicine is a complex system composed of multiple components and factors. The diversity and complexity of its chemical components are the material basis for its curative effect. Establish a modern quality control system in line with the characteristics of traditional Chinese medicine to overcome the problems of quality analysis and evaluation of traditional Chinese medicine. Improving the existing quality control methods of traditional Chinese medicine has become a subject of active research. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 张铁军刘素香韦辉刘毅韩丰年何立新刘向
Owner SHIJIAZHUANG DONGFANG PHARMA
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