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Houttuynia cordata aerial stem tissue culture rapid propagation method

A tissue culture and grassland technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve high seedling rate, large reproduction coefficient, and the effect of industrialized seedling raising

Inactive Publication Date: 2013-01-09
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the deficiencies of the prior art, to provide a method for culturing the aboveground stem nodes of Houttuynia cordata that is simple, feasible, fast-growing, and effectively reduces the pollution rate.

Method used

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  • Houttuynia cordata aerial stem tissue culture rapid propagation method
  • Houttuynia cordata aerial stem tissue culture rapid propagation method
  • Houttuynia cordata aerial stem tissue culture rapid propagation method

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Embodiment 1

[0030] Disinfection treatment: First, select healthy and tender Houttuynia cordata plants in the field, cut off the jointed aboveground stems and rinse them with clean water, and perform the first disinfection treatment with 0.1% mercury chloride for 12 minutes; Soak in the mixture for 24 hours, and the concentrations of streptomycin and penicillin sodium in the mixture are both 0.5g / L; soak in 70% alcohol for 30 seconds, and then perform a second disinfection treatment in 0.1% mercury chloride for 10 minutes; finally rinse with sterile water for 30 seconds , take out the above-ground stems and place them on the sterilized absorbent paper, the pollution rate can be reduced to 0% ( figure 1 );

[0031] Adventitious bud induction culture: Under sterile conditions, inoculate the sterilized 5-10cm long stem section (with 1-2 axillary buds) on the adventitious bud induction medium for induction culture; medium formula: MS+0.5 mg / L NAA+2.0mg / L 6-BA; after culturing for 14 days unde...

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Abstract

The invention discloses a houttuynia cordata aerial stem tissue culture rapid propagation method, through processes such as disinfection, adventitious bud inducing, propagation, cutting section culture, rooting and transplant, rapid propagation of a houttuynia cordata aerial stem tissue is achieved successfully, and industrialized seedling culture and high-efficiency production are achieved; culture and growth are controlled artificially, the method is not affected by natural conditions, the material drawing quantity is small, a culture material is economical, a growing period is short, a propagation coefficient is high, and the management is convenient. Besides, the problem that the pollution rate is high when primary vaccination of houttuynia cordata is conducted is solved; the plantingsurvival rate is high in aerial stem culture, a procedure is simple and practical, and the problems that houttuynia cordata test-tube plantlets in slow in propagation period and grow slowly are solved effectively; the propagation coefficient reaches 4.98 times, the rapid production period is 50d, GA3 is added in the test-tube plantlets, propagation bud clumps with high length can be acquired, andpropagation can be achieved; and at the same time, the problem that the cost of large-scale production is high is solved.

Description

technical field [0001] The invention relates to a method for plant in vitro culture and rapid propagation, in particular to a method for rapid propagation of houttuynia cordata aboveground stem tissue culture. Background technique [0002] The scientific name of Houttuynia cordata is Houttuynia genus, which belongs to the genus Houttuyniaceae. It is a perennial herbaceous plant. It is native to my country and is mainly distributed in the central, southeast and southwestern provinces of my country. North reaches Shaanxi and Gansu, especially Sichuan, Hubei, Hunan, Jiangsu and other provinces. The tender stems and leaves and underground stems are used as vegetables, and the whole plant can be used fresh or dried as medicine. It has been officially identified by the Ministry of Health as one of the resources with great development potential that is "both medicine and food". [0003] Houttuynia cordata has always been a wild plant. It has been artificially cultivated since the e...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 钟军熊兴耀李炎林周小云李爱华黄放张寒
Owner HUNAN AGRICULTURAL UNIV
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