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Method for identifying pure cordyceps sinensis powder

A technology of pure cordyceps sinensis powder and cordyceps sinensis powder, which is applied in the field of medicinal material identification, can solve the problems such as difficulty in distinguishing authenticity, inability to detect impurities, inability to identify counterfeit products, etc., and achieves the effects of protecting benefits, low cost, and difficulty in interference.

Active Publication Date: 2013-01-23
QINGHAI SPRING MEDICINAL RESOURCES TECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The pure Cordyceps sinensis powder that is currently circulated and sold in the market is made into a certain dosage form, so it is difficult to distinguish the authenticity from the fake, although the means of testing equipment can be used to prove the existence of some markers of Cordyceps sinensis, such as the content of cordycepin and adenosine , but these markers are easy to buy, and they are not expensive, and they can be used as additives in theory, so physical and chemical tests can neither detect impurities nor distinguish fakes

Method used

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  • Method for identifying pure cordyceps sinensis powder
  • Method for identifying pure cordyceps sinensis powder
  • Method for identifying pure cordyceps sinensis powder

Examples

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Embodiment Construction

[0033] 5. Experimental methods and parameters:

[0034] 1. Experimental samples:

[0035] Cordyceps slices, commercially available Jicao brand pure Cordyceps superfine powder, starch superfine powder, starch superfine powder and pure Cordyceps superfine powder mixture.

[0036] 2. Dyeing agent

[0037] Fast green dyeing solution: Sinopharm Group Chemical Reagent Co., Ltd.; safranin dyeing solution: Sinopharm Group Chemical Reagent Co., Ltd.; eosin dyeing solution: Shenyang Xinguang Chemical Factory; hematoxylin dyeing solution: Shenyang Xinguang Chemical Factory; Steiner's solution: Beijing Chemical plant; iodine: Nanjing Xinhuayuan Chemical Co., Ltd., prepared dye solution according to conventional methods.

[0038] 3. Instruments or other utensils

[0039] Binocular biological microscope, camera adapter, Canon SLR camera. Alcohol lamps, glass slides, coverslips, staining racks, cedar oil, absolute ethanol, xylene, Canadian gum, lens cleaning paper, distilled water and ot...

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Abstract

The invention belongs to the field of identification of medicinal materials, and in particular relates to a method for identifying pure cordyceps sinensis powder through microscopic staining and aims to solve the technical problem that the pure cordyceps sinensis powder circulated and sold in the market is difficult to distinguish. According to the technical scheme, the invention provides the method for accurately identifying the cordyceps sinensis powder. The method comprises the following steps of: taking cordyceps sinensis powder to be detected, and dyeing the cordyceps sinensis powder to prepare a smear; and performing microscopic observation on the prepared smear under a microscope, wherein if the specific structure of cordyceps sinensis can be clearly observed on each smear, obvious blue spots under iodine solution dyeing are absent, and obvious plant tissues are not observed, the powder can be judged to be pure cordyceps sinensis powder, otherwise the powder is impure cordyceps sinensis powder. According to the method, fine decoction piece powder can be clearly identified, the substances which do not belong to cordyceps sinensis are distinguished; and the method is low in cost, high in efficiency and high in accuracy, is hardly interfered by various added ingredients and has good application prospect in the field.

Description

[0001] This application is a divisional application of an invention patent application with an application date of April 21, 2010, an application number of 201010151881.9, and an invention title of "Method for Differentiating Winter, Spring and Summer Grass Powder by Microscopic Staining". technical field [0002] The invention belongs to the field of medicinal material identification, and in particular relates to a method for identifying cordyceps sinensis powder by microscopic staining. Background technique [0003] In the traditional medicine of our country, Cordyceps sinensis has always been a high-grade tonic, and its medicinal status is very prominent. It is also known as the "three major tonics" together with ginseng and velvet antler. With the in-depth research in various related disciplines, there have been reports of new species and new distributions of Cordyceps in recent years. Confirmed the efficacy of Cordyceps sinensis. Due to the special growth environment, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/88G01N1/30
Inventor 张雪峰徐丽
Owner QINGHAI SPRING MEDICINAL RESOURCES TECHNOLOGY CO LTD
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