Method for producing laver antihypertensive peptides

A technology of antihypertensive peptide and seaweed, which is applied in the field of preparation of laver antihypertensive peptide, can solve problems such as side effects, achieve strong operability, reasonable process, and reduce losses

Inactive Publication Date: 2013-02-13
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemically synthesized drugs are commonly used in the treatment of hypertension, but long-term use of synthetic drugs by hypertensive patients will produce various side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] A preparation method of laver antihypertensive peptide, characterized in that: through the following process

[0012] Select laver dry powder as raw material, add water according to the proportion of 8% (W / V), homogenate, adjust the pH to 7.5 with ammonia water, adjust the temperature to 50°C, add alkaline protease, the enzyme dosage of alkaline protease is per gram of laver Add 3000 enzyme activity units (U) to the weight of the dry powder, stir, and enzymolyze for 6 hours; after the enzymolysis is completed, heat and boil for 10 minutes, perform enzyme inactivation treatment, and centrifuge to obtain a supernatant; the obtained supernatant is measured by molecular weight The ultrafiltration membrane with a cut-off value of 2000 is subjected to ultrafiltration treatment, and the filtrate is collected; the filtrate is concentrated under reduced pressure at a temperature of 55°C to a solid content of 50%, freeze-dried, and pulverized to obtain laver depressurization pept...

Embodiment 2

[0014] A preparation method of laver antihypertensive peptide, characterized in that: through the following process

[0015] Choose laver dry powder as raw material, add water according to the proportion of 5% (W / V), homogenate, adjust the pH to 7.5 with ammonia water, adjust the temperature to 48°C, add alkaline protease, the enzyme dosage of alkaline protease is per gram of laver Add 3000 enzyme activity units (U) to the weight of the dry powder, stir, and enzymolyze for 5 hours; after the enzymolysis is completed, heat and boil for 12 minutes, perform enzyme-inactivating treatment, and centrifuge to obtain a supernatant; the obtained supernatant is measured by molecular weight The ultrafiltration membrane with a cut-off value of 2000 is subjected to ultrafiltration treatment, and the filtrate is collected; the filtrate is concentrated under reduced pressure at a temperature of 58°C to a solid content of 55%, freeze-dried, and pulverized to obtain the laver depressurization ...

Embodiment 3

[0017] A preparation method of laver antihypertensive peptide, characterized in that: through the following process

[0018] Select laver dry powder as raw material, add water according to the ratio of 10% (W / V), homogenate, adjust the pH to 7.5 with ammonia water, adjust the temperature to 45°C, add alkaline protease, the enzyme dosage of alkaline protease is per gram of laver Add 3000 enzyme activity units (U) to the weight of the dry powder, stir, and enzymolyze for 7 hours; after the enzymolysis is completed, heat and boil for 15 minutes, perform enzyme inactivation treatment, and centrifuge to obtain a supernatant; the obtained supernatant is measured by molecular weight The ultrafiltration membrane with a cut-off value of 2000 is subjected to ultrafiltration treatment, and the filtrate is collected; the filtrate is concentrated under reduced pressure at a temperature of 60°C to a solid content of 45%, freeze-dried, and pulverized to obtain laver depressurization peptide....

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Abstract

The invention relates to a method for producing laver antihypertensive peptides. The method is that dry laver powder is selected as a raw material, is added with water and homogenized, the pH is regulated to 7.5, and alkaline protease is added at the temperature of 45-50 DEG C for enzymolysis for 5-7 hours; the alkaline protease is deactivated, and supernatant liquor is obtained through centrifugation; the supernatant liquor is filtered through an ultrafitration membrane which takes the molecular weight 2000 as the retention value, and filtrate is collected; and the filtrate is subjected to reduced-pressure condensation until the solid content is 45%-55%, and is subjected to freeze drying and grinding so as to obtain the laver antihypertensive peptides. The method for producing laver antihypertensive peptides is reasonable in technology, is simple to produce and has strong operability.

Description

technical field [0001] The invention relates to the preparation of peptides, in particular to a preparation method of laver antihypertensive peptide. Background technique [0002] Hypertension is a dangerous and frequently-occurring disease. The number of people suffering from hypertension in the world has exceeded 500 million. At the same time, it is the most important pathogenic factor of arteriosclerosis, coronary heart disease, heart and kidney failure. The treatment of hypertension is a global medical a daunting task facing the community. At present, chemically synthesized drugs are commonly used in the treatment of hypertension, but long-term use of synthetic drugs by hypertensive patients will produce various side effects. As people's awareness of their own health continues to increase, medical staff and patients prefer non-chemical synthetic drug therapy. [0003] Antihypertensive peptides, also known as angiotensin converting enzyme inhibitory peptides (ACEIPs for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K4/08C07K1/34
Inventor 刘新刘昌衡孙永军袁文鹏贾爱荣胡炜张绵松夏雪奎张永刚孟秀梅鞠文明
Owner SHANDONG HOMEY AQUATIC DEV
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