Preparation method of quantum dot electrochemical luminescence electrode based on deoxyribonucleic acid (DNA) charge transfer

A light-emitting electrode and charge transfer technology, applied in the field of electrochemiluminescence, can solve rare problems and achieve the effect of mild conditions and good electrochemiluminescence performance

Inactive Publication Date: 2014-12-03
BEIJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the current research reports, most of them use a certain method to directly connect quantum dots on the electrode as a detection sensor, and there are few reports on the influence of the change of the luminescence signal of quantum dots on DNA charge transfer.

Method used

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  • Preparation method of quantum dot electrochemical luminescence electrode based on deoxyribonucleic acid (DNA) charge transfer
  • Preparation method of quantum dot electrochemical luminescence electrode based on deoxyribonucleic acid (DNA) charge transfer
  • Preparation method of quantum dot electrochemical luminescence electrode based on deoxyribonucleic acid (DNA) charge transfer

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Experimental program
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Embodiment 1

[0029] 1) Hybridization of double-stranded dsDNA: Melt the two single-stranded ssDNA stock solutions frozen in a shaker at room temperature. The sequences of the two single-stranded ssDNA are as follows: 5'-SH-GACTGACCTCGGACG and 5'-NH 2 -CGTCCGAGGTCAGTC. Mix the ssDNA stock solution with the same molar number respectively, place the mixed solution in a constant temperature water bath at 90°C for 5 minutes, and cool it to room temperature in a natural environment to form hybridized double-stranded dsDNA, and store it in a freezer at -20°C for later use;

[0030] 2) Preparation of 1mM 6-mercaptohexan-1-ol MCH: first prepare 5% glycerol phosphate buffer: use 5.0mM pH=7.0 phosphate buffer to make up the volume, after the volume is set up, vibrate evenly on the shaker, and save it for later use; Then prepare 1mM MCH: use the configured glycerol phosphate buffer solution to make up the volume, after the volume is set up, vibrate evenly on the oscillator, and save it for later use; ...

Embodiment 2

[0050] Step 1) 2) 3) 4) 5) 6) 7) 8) 9) 10) with embodiment 1;

[0051] 11) Rinse the electrode and place it in 0.1M pH=7.4 PB+0.1M K 2 S 2 o 8 In +0.1M KCl solution, the modified electrode was used as the working electrode, the platinum wire was used as the counter electrode, and the calomel electrode was used as the reference electrode for cyclic voltammetry scanning. The potential scanning range was 0.8 V ~ -1.8 V, and the scanning speed was is 100 mV / s.

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Abstract

The invention provides a preparation method of quantum dot electrochemical luminescence electrode based on deoxyribonucleic acid (DNA) charge transfer and belongs to the field of electrochemical luminescence. A gold electrode which is polished smoothly and cleaned cleanly is activated in sulfuric acid by means of cyclic voltammetry; DNA is dropwise added on the surface of the gold electrode, 6-Mercaptohexan-1-ol (MCH) is used for sealing, and then DNA / Au electrode is immersed in mixed solution of cadmium selenide quantum dot, 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxy succinimide (NHS) and carries out strenuous vibration reaction for 30 minutes. The modified gold electrode is used as a working electrode, a platinum filament is used as a counter electrode, a saturated calomel electrode is used as a reference electrode, electrolyte is scanned by means of the cyclic voltammetry, a photomultiplier (PMT) is used to collect optical signals, electric resistance of the PMT is arranged to be one kilohm, an electric potential scanned area is 0.8-1.8 volt, and scanning speed is 100 mV / s. The quantum dot electrochemical luminescence electrode can be used to research charge transfer functions of the DNA visually and has good electrochemical luminescence performance and conditions are moderate.

Description

technical field [0001] The invention relates to a preparation method of a double-stranded DNA electrochemical luminescence sensor directly connected to a capped quantum dot on the surface of a gold electrode, and belongs to the field of electrochemiluminescence. Background technique [0002] In recent years, because the electrochemiluminescence method not only has the advantages of high chemiluminescence sensitivity, wide linear range, convenient observation and simple instrument, but also has the advantages of strong controllability and good selectivity of electrochemical analysis, it has been applied in pharmaceutical analysis and environmental analysis. , immunoassay and DNA analysis. [0003] At present, nanotechnology has widely penetrated into chemistry, biology, medicine, materials, electronics and other disciplines, forming a complete system integrating cutting-edge basic disciplines and high technology. Semiconductor nanocrystals, namely quantum dots, are a new typ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 李萌鲁理平
Owner BEIJING UNIV OF TECH
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