Kit for detecting mRNA expression quantity of M BCR fusion gene

Abstract

The invention relates to a fluorescent quantitative PCR (Polymerase Chain Reaction) diagnosis kit for detecting mRNA of an M BCR fusion gene, belonging to the field of biotechnology. The kit comprises a detection primer, a fluorescent probe, a cDNA first-chain synthesis reagent, a fluorescent quantitative PCR mixed liquor, negative control and positive control, wherein the detection primer and the fluorescent probe comprise an M BCR gene primer, a reference gene ABL primer and a Taqman fluorescent probe. The kit can effectively detect the M BCR fusion gene forms, such as e14a3, e13a3, e14a2 and e13a2. The M BCR fusion gene is a gene mark of malignant clone of pluripotential hemopoietic stem cells, is a typical molecular marker of chronic granulocytic leukemia and is related to inhibition of the apoptosis of the leukemic cell. The fluorescent quantitative PCR technology with higher sensitivity and specificity is used for detecting the mRNA level of the M BCR gene, and the specificity and the sensitivity of the detection result both are improved remarkably. The kit provided by the invention provides a brand-new rapid, simple and convenient gene diagnosis technology for making a therapeutic regimen for the patients with chronic granulocytic leukemia, and predicting prognosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for detecting the expression level of M BCR fusion gene mRNA. Background technique [0002] In addition to 90% of the blood cells of patients with chronic myelocytic leukemia (CML) being positive for Ph1 chromosome, they are often accompanied by t(9;22)(q34;q11) translocation, which is located on the long arm of chromosome 9 The abl proto-oncogene is translocated to the breakpoint concentrated region bcr on the long arm of chromosome 22, forming the MBCR fusion gene. The fusion gene is responsible for encoding the P210 protein that enhances the activity of tyrosine kinase, thereby changing the tyrosine phosphorylation level of various proteins in the cell and the function of the microfilament motor protein in the cell, resulting in abnormal signal transduction in the cell and causing the cell to lose the ability to control the surrounding environment. The responsiveness of the...

AI Technical Summary

Problems solved by technology

[0004] Real-time fluorescent quantitative PCR (Polymerase Chain Reaction, Polymerase Chain Reaction) technology has realized the leap from qualitative to real quantitative PCR, and provided an effective detection tool for the quantitative detection of human disease genes, which is comparable to ordinary PCR. It has stronger specificity and sensitivity than other methods, and has the advantages of rapid detection and reduced pollution. However, there is no real-time fluorescent quantitative PCR method for detecting M BCR fusion genes.

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