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One-step rapid seedling method for tender stem segment of malus komarovii inside test tube

An in-vitro, Begonia technology, applied in the field of plant reproduction, can solve the problems of low survival rate of wild resources by felling, excavation, transplanting, and large consumption of wild resources for reproduction, and achieves high rooting rate, fast growth, and operable strong effect

Active Publication Date: 2013-03-20
TONGHUA NORMAL UNIV
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AI Technical Summary

Problems solved by technology

[0005] In reality, there are few wild resources of Hawthorn Begonia, conventional methods such as cuttings and seeds consume a lot of wild resources, rooting and germination are difficult, and the survival rate of transplanting is low, which leads to severe logging and digging of wild resources. Reasonable methods are needed for artificial propagation

Method used

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  • One-step rapid seedling method for tender stem segment of malus komarovii inside test tube

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Embodiment Construction

[0015] 1 Materials and methods

[0016] 1.1 Materials and handling

[0017] In late October, the branches of hawthorn and crabapple were collected, and the branches were cut into sections (30 cm in length), wrapped in plastic bags, placed in a freezer for low-temperature dormancy, and the temperature was controlled at -5 to 0 °C, and taken out after 45 days. Hydroponics promotes the germination of its dormant buds. When the dormant buds germinate and grow to 3.00 cm, cut the dormant buds, remove the young leaves and petioles, rinse with 70% ethanol (volume fraction) for 30 s, transfer to saturated sodium hypochlorite solution for 10 min, and rinse with sterile water for 7 min. The second time, dry the surface moisture with sterile filter paper, remove the tissue poisoned by ethanol and sodium hypochlorite, and cut it into 1 leaf and 1 segment as explants for later use.

[0018] 1.2 Hawthorn Begonia stems take root while axillary buds germinate and grow, establishment of rapi...

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Abstract

The invention relates to plant propagation, namely a one-step rapid seedling method for a tender stem segment of malus komarovii inside a test tube. The method comprises the steps of (1) collecting branches of the malus komarovii in the last ten days of October, carrying out dormancy at low temperature; promoting a dormant bud to germinate by water culture, and cutting the processed branches into segments, each of which contains a leaf, as explants for use; (2) enabling the malus komarovii to root, wherein the culture medium for growth of an axillary bud of the stem segment is culture medium+6-BA (0.07-0.09mg.L-1)+IBA (0.02-0.04mg.L-1)+GA3(0.09-0.13mg.L-1). By adopting the one-step rapid seeding method disclosed by the invention, rooting of the tender stem segment of the malus komarovii inside the test tube is facilitated, and germination of the axillary bud is facilitated at the same time; the method is high in rooting percentage, rapid in germination speed of the axillary bud, and strong in maneuverability; just one low-cost culture medium is utilized from inoculation of the stem segment to germination; and the one-step rapid seeding method belongs to a one-step seeding method, and can meet the requirement of industrialized seedling production.

Description

[0001] technical field [0002] The invention relates to a kind of plant propagation, that is, a method for one-step rapid seedling growth in test tubes of tender stems of hawthorn and crabapple. Background technique [0003] In the prior art, Hawthorn Begonia Malus komarovii (Sarg.) Rehd. Also known as thin-leaf hawthorn and mountain apple, it is a small deciduous tree in the subgenus Malus of the Rosaceae family. The fruit contains protein, fat, sugar, ash, vitamin C, malic acid, riboflavin, carotene and Calcium, phosphorus, iron, etc. The fruit can be eaten raw, and can also be used to make wine, juice and jam. The tree is short and cold-resistant. It is the best dwarf rootstock for apples, pears, and catalpas. The flowers are beautiful, the smell is delicate, the fruit is gorgeous, and the fruit-hanging period is long. It can be used as a landscape tree. [0004] Hawthorn crabapple is very rare in Changbai Mountains, and its distribution area is very narrow. It only ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 顾地周朱俊义姜云天郭志欣杨丽娟梁宇陆爽潘雨张学士
Owner TONGHUA NORMAL UNIV
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