Modeling processing method of non-alcoholic fatty liver mouse model

A non-alcoholic and fatty liver technology, applied in the field of new technology of non-alcoholic fatty liver mouse model, can solve the problems of weak constitution, sudden death of model animals, prolonging the time of modeling, etc., and achieves a simple method, easy implementation, low cost effect

Inactive Publication Date: 2013-03-20
GUIZHOU UNIV
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Problems solved by technology

At the beginning, the appetite of the mice was relatively large, but because the content of lard is too high, it is easy to produce a sense of satiety and greasy feeling, which leads to the loss of appetite of the mice and a significant decrease in food intake, which prolongs the time for modeling
There is another disadvantage of the

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  • Modeling processing method of non-alcoholic fatty liver mouse model
  • Modeling processing method of non-alcoholic fatty liver mouse model

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Embodiment Construction

[0016] In order to prove the effectiveness of the modeling method of this kind of non-alcoholic fatty liver model mice, the following experiments were therefore carried out:

[0017] Comparison of serum TC and TG levels between model mice and normal mice:

[0018] 1. Purchase 24 Kunming clean-grade mice with animal numbers, half male and half male, weighing about 18g. After purchasing the laboratory, first carry out adaptive feeding for about 3 days to a week, depending on the condition of the mice. If you are in good condition, you can get used to it for 3 days, otherwise it will be extended for 7 days. If the condition is not good at 7 days (referring to not being active enough), it will be discarded and not put into the experiment, because Kunming mice are more active and aggressive, especially male mice. Cage bedding and water were changed every day, and basal feed was fed during this period. After adapting to the environment, the animals were randomly divided into two ...

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Abstract

The invention discloses a modeling method of a non-alcoholic fatty liver mouse model, and the method is characterized by consisting of the following steps that (1) a healthy mouse is caught and first adaptively raised for 3 to 7 days in an animal house, a backing material and clean water in a mouse cage are changed every day, and basic fodder is fed during that period of time; (2) after adaptive raising, composite high-fat fodder is fed every day; (3) 5 percent CC14 liquid is injected into the abdominal cavity of the mouse two weeks later, the liquid carbon tetrachloride is diluted to 5 percent emulsion with deionized water, and the liquid which is injected into the abdominal cavity shall be newly prepared; the abdominal cavity injection state continues for the 28th day, and meanwhile the composite high-fat fodder is fed; and the model is obtained. The modeling method has the advantages of short time, high modeling success rate, extremely low mortality rate and low cost.

Description

technical field [0001] The invention relates to a modeling technique of an animal model, in particular to a novel technique of a nonalcoholic fatty liver mouse model. Background technique [0002] With the improvement of people's living standards, the dietary structure has changed significantly, and the incidence of fatty liver disease (also known as fatty liver), which is called "rich man's disease", has increased significantly, especially in Asia. It has become the second most common liver disease after viral hepatitis. Fatty liver is caused by a variety of factors. The main pathological changes are in the hepatic lobules. It is one of the common diffuse liver diseases. Clinically, fatty liver is divided into alcoholic fatty liver disease (AFLD) and nonalcoholic fatty liver disease (NAFLD) according to whether there is excessive alcohol intake. Non-alcoholic fatty liver disease is more complicated, if not controlled in time, it will lead to inflammation and liver fibrosi...

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Application Information

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IPC IPC(8): A01K67/02A61K49/00
Inventor 陈祥盛薛琰肖思卫
Owner GUIZHOU UNIV
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