Tissue culture method of gelidium amansii

A technology of tissue culture and agaric, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as long time, and achieve the effect of improving the survival rate and growth speed of tissue culture

Inactive Publication Date: 2013-03-27
溧阳市天目湖保健品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

What is used in this patent document is the common seaweed medium PES enriched medium, and it ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take the following steps for tissue culture of Geliflower:

[0022] (1) Preparation and disinfection of raw materials

[0023] Take the main branches or branches of agaricus in good growth condition as raw materials, and process them. When cleaning, use an ultrasonic cleaner to clean the surface of agaricus, then rinse with clean water, and then cut the agaricus into 2mm-sized blocks And soak it in 0.1% mercuric chloride solution for 2 minutes, then use antibiotic sterilization procedure, and then soak it in sterile water 5 times, 10 minutes each time;

[0024] (2) Preparation and inoculation of seedling medium

[0025] The formula of seedling medium is as follows: NaCl 30g / L, MgSO 4 .7H 2 O 10g / L, EDTA-Fe25mg / L, K 2 HPO 4 80mg / L,H 3 BO 3 30mg / L, MnCl 2 20mg / L, ZnCl 2 12mg / L, Co(NO 3 ) 2 .6H 2 O 1.5mg / L, zeatin 2.0mg / L, 5-aminolevulinic acid 1.0mg / L, agar 10g / L;

[0026] Inoculate the small pieces of Geliflower obtained in step (1) onto the seedling medi...

Embodiment 2

[0037] Take the following steps for tissue culture of Geliflower:

[0038] (1) Preparation and disinfection of raw materials

[0039] Take the main branches or branches of agaricus in good growth condition as raw materials, and process them. When cleaning, use an ultrasonic cleaner to clean the surface of agaricus, then rinse it with clean water, and then cut the agaricus into 3mm-sized blocks And soak it in 0.1% mercuric chloride solution for 3 minutes, then use antibiotic sterilization procedure, and then soak it in sterile water 5 times, 10 minutes each time;

[0040](2) Preparation and inoculation of seedling medium

[0041] The formula of seedling medium is as follows: NaCl 25g / L, MgSO 4 .7H 2 O 8g / L, EDTA-Fe20mg / L, K 2 HPO 4 70mg / L,H 3 BO 3 20mg / L, MnCl 2 15mg / L, ZnCl 2 10mg / L, Co(NO 3 ) 2 .6H 2 O 1mg / L, zeatin 1.5mg / L, 5-aminolevulinic acid 0.5mg / L, agar 10g / L;

[0042] Inoculate the small pieces of Geliflower obtained in step (1) onto the seedling mediu...

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Abstract

The invention provides a tissue culture method of gelidium amansii. The method mainly comprises the steps of: selection preparation and disinfection of raw materials, cultivation of seedlings, and cultivation of plants. The invention also discloses specific formulas of a seedling cultivation medium and a plant cultivation medium, and cultivation conditions of the seedling culture and the plant cultivation.

Description

technical field [0001] The invention relates to a method for breeding Geliflower, in particular to a method for tissue culture of Geliflower. Background technique [0002] Gemweed is a perennial algae. The algal body is upright, multi-branched, and the lower part has a pseudoroot-like holder attached to the rocks on the shallow seabed, generally 20-30 cm high, purple or deep red. The algal body is composed of epidermal cells, surface layer and pith. The epidermal cells are closely arranged, the cortex and pith are relatively loose, and the cells are full of colloid. Lithium cauliflower is dioecious, and the algae have female gametophytes, male gametophytes and tetrasporophytes, mainly sexual reproduction, and the reproductive process is relatively complicated. The algal body of Geliflower contains a large amount of polysaccharide compounds of galactose gum, which is the main raw material for making agar (commonly known as agar). [0003] Since the reproductive method of G...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 彭江晨芮旭婷
Owner 溧阳市天目湖保健品有限公司
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