Tissue culture method of gelidium amansii
A technology of tissue culture and agaric, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as long time, and achieve the effect of improving the survival rate and growth speed of tissue culture
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Embodiment 1
[0021] Take the following steps for tissue culture of Geliflower:
[0022] (1) Preparation and disinfection of raw materials
[0023] Take the main branches or branches of agaricus in good growth condition as raw materials, and process them. When cleaning, use an ultrasonic cleaner to clean the surface of agaricus, then rinse with clean water, and then cut the agaricus into 2mm-sized blocks And soak it in 0.1% mercuric chloride solution for 2 minutes, then use antibiotic sterilization procedure, and then soak it in sterile water 5 times, 10 minutes each time;
[0024] (2) Preparation and inoculation of seedling medium
[0025] The formula of seedling medium is as follows: NaCl 30g / L, MgSO 4 .7H 2 O 10g / L, EDTA-Fe25mg / L, K 2 HPO 4 80mg / L,H 3 BO 3 30mg / L, MnCl 2 20mg / L, ZnCl 2 12mg / L, Co(NO 3 ) 2 .6H 2 O 1.5mg / L, zeatin 2.0mg / L, 5-aminolevulinic acid 1.0mg / L, agar 10g / L;
[0026] Inoculate the small pieces of Geliflower obtained in step (1) onto the seedling medi...
Embodiment 2
[0037] Take the following steps for tissue culture of Geliflower:
[0038] (1) Preparation and disinfection of raw materials
[0039] Take the main branches or branches of agaricus in good growth condition as raw materials, and process them. When cleaning, use an ultrasonic cleaner to clean the surface of agaricus, then rinse it with clean water, and then cut the agaricus into 3mm-sized blocks And soak it in 0.1% mercuric chloride solution for 3 minutes, then use antibiotic sterilization procedure, and then soak it in sterile water 5 times, 10 minutes each time;
[0040](2) Preparation and inoculation of seedling medium
[0041] The formula of seedling medium is as follows: NaCl 25g / L, MgSO 4 .7H 2 O 8g / L, EDTA-Fe20mg / L, K 2 HPO 4 70mg / L,H 3 BO 3 20mg / L, MnCl 2 15mg / L, ZnCl 2 10mg / L, Co(NO 3 ) 2 .6H 2 O 1mg / L, zeatin 1.5mg / L, 5-aminolevulinic acid 0.5mg / L, agar 10g / L;
[0042] Inoculate the small pieces of Geliflower obtained in step (1) onto the seedling mediu...
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