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New use of synechocystis protein in paddy rice property improvement

A protein and rice technology, applied in the direction of plant genetic improvement, application, algae/bryopeptide, etc., can solve unclear problems

Inactive Publication Date: 2013-03-27
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether this mechanism of action is effective in monocotyledonous plants represented by rice remains unclear

Method used

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  • New use of synechocystis protein in paddy rice property improvement
  • New use of synechocystis protein in paddy rice property improvement
  • New use of synechocystis protein in paddy rice property improvement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Overexpression of slr1512 gene improves chlorophyll content and heading rate of transgenic rice

[0031] 1. Obtaining and identifying rice plants overexpressing slr1512

[0032] 1. Construction of overexpression slr1512 gene vector

[0033] Synechocystis PCC 6803 in the logarithmic growth phase is extracted (the public can obtain it from the Institute of Botany, Chinese Academy of Sciences, and the non-patent literature that has recorded this material is: Zhang LF et al.Proteomic analysis of plasma membranes of cyanobacterium Synechocystis sp. Strain PCC 6803 in response to high pH stress. J Proteome Res. 2009) cell DNA was used as a template to amplify the gene slr1512 by PCR. No high homology gene was found in the transgenic recipient plant rice. After the PCR amplification product was separated and recovered by electrophoresis, it was connected to the gene cloning vector pMD18 and transformed into Escherichia coli, and the plasmid sequencing analysis of t...

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Abstract

The invention discloses a new use of a synechocystis protein in paddy rice property improvement, provides an application of a protein having a structure represented by sequence 2 in a sequence table in the improvement of the plant chlorophyll content and / or the plant heading rate, and also provides an application of a gene encoded by the protein having a structure represented by the sequence 2 in the sequence table in the improvement of the plant chlorophyll content and / or the plant heading rate. Transgenic experiments that an slr1512 gene is introduced into paddy rice prove that the chlorophyll content and the heading rate of the slr1512 gene transferred paddy rice are obviously improved, so the slr1512 gene and products encoded thereby are of great theoretical and practical significance to the cultivation of photosynthesis enhanced new species of crops, and can be used for cultivating agricultural high-yield plant varieties.

Description

technical field [0001] The invention relates to a new application of a Synechocystis protein in improving rice traits in the field of biotechnology. Background technique [0002] In recent years, due to the shortage of energy, the development of alternative energy sources based on food has exacerbated the food shortage. At the end of 2008, world cereal stocks had fallen to 405 million tonnes, the lowest level in almost 25 years. Protests and riots have occurred in 37 countries, including Haiti, the Philippines and Egypt, over sharp increases in food prices. Therefore, it is of great strategic significance to increase crop yields, ensure food supply and price stability, and maintain world peace and stability. In my country, the area of ​​arable land is decreasing day by day, so increasing the yield per unit area of ​​major crops is the most realistic, effective and fundamental way to solve the food problem in our country. Since more than 90% of the dry matter in crops come...

Claims

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Application Information

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IPC IPC(8): C07K14/405C12N15/31C12N15/63A01H5/00
Inventor 黄芳杨浩萌赵乐薛哲勇周荷益漆小泉
Owner INST OF BOTANY CHINESE ACAD OF SCI
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