Soybean flowering gene ft2a-1 and protein coded by the gene

A technology of ft2a-1 and encoding protein, applied in the field of soybean flowering gene ft2a-1 and its encoded protein

Inactive Publication Date: 2013-03-27
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how the mutation of the GmFT2a gene affects the soybean growth period (flowering period and maturity

Method used

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  • Soybean flowering gene ft2a-1 and protein coded by the gene
  • Soybean flowering gene ft2a-1 and protein coded by the gene
  • Soybean flowering gene ft2a-1 and protein coded by the gene

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Experimental program
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Effect test

specific Embodiment approach 1

[0011] Embodiment 1: The gene sequence of the soybean flowering gene ft2a-1 in this embodiment is shown in the sequence table Seq ID No: 1.

[0012] In this embodiment, the GmFT2a gene sequence in different varieties is studied, and the ft2a-1 gene is determined, which is closely related to the growth period of soybean.

[0013] In this embodiment, the GmFT2a gene and ft2a-1 gene are expressed in Arabidopsis thaliana through genetic transformation means, and it is verified that the ft2a-1 gene of the present invention is significantly weaker in promoting flowering than the soybean flowering gene GmFT2a .

specific Embodiment approach 2

[0014] Embodiment 2: The amino acid sequence of the encoded protein of the soybean flowering gene ft2a-1 in this embodiment is shown in Seq ID No: 2 in the sequence table.

[0015] Verify effect of the present invention by following test:

[0016] (1) Acquisition of gene ft2a-1 sequence

[0017] 1. Using the leaves of soybean variety K3 as materials, extract the total RNA from the leaves with the operation manual of the TRIzol kit purchased from Invitrogen; 2. Treat the total RNA extracted in step 1 with DNase I; The total RNA was used for cDNA synthesis, and the cDNA synthesis was performed according to the manual of the BD SMARTTM RACE cDNA Amplification Kit purchased from BD BiosciencesClontech Company to obtain cDNA; 4. The obtained cDNA was used as a template to amplify with F1 and R1 primers For the ft2a-1 gene, the PCR reaction conditions were as follows: pre-denaturation at 94°C for 5 min, denaturation at 94°C for 30 s, annealing at 56°C for 30 s, extension at 72°C fo...

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Abstract

A Soybean flowering gene ft2a-1 and a protein coded by the gene. The invention relates to the soybean flowering gene ft2a-1 and protein coded by the gene. The invention aims to provide the soybean flowering gene ft2a-1 and the protein coded by the gene. The soybean flowering gene ft2a-1 provided by the invention has a gene sequence shown as a Seq ID No:1 in the sequence table. An amino acid sequence of the protein coded by the soybean flowering gene ft2a-1 is shown as a Seq ID No:2 in the sequence table. According to Arabidopsis genetic transformation verification, it illustrates that the ft2a-1 gene has obviously decreased function of promoting flowering compared with an FT2a gene. The invention can be applied to soybean gene engineering.

Description

technical field [0001] The invention relates to soybean flowering gene ft2a-1 and its encoded protein. Background technique [0002] Soybeans provide human beings with important vegetable proteins and oils. Worldwide, soybeans are cultivated in a wide range of regions ranging from high latitudes in northern Europe, Sweden and North America to Canada, and south to Brazil and Argentina. It is closely related to soybean photoperiod and growth period genes or quantitative trait loci (Quantitative Trait Locus, QTL). Breeding high-quality soybean varieties with wide ecological adaptability is the fundamental way to realize high-yield, high-quality and high-efficiency soybean industry. However, conventional breeding has made slow progress in the selection of widely-adapted varieties. The use of molecular breeding methods is expected to regulate the photoperiod and growth period of soybean in a targeted manner and speed up the selection of widely-adapted varieties. The results of...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415
Inventor 孔凡江刘宝辉
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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