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Method for quickly obtaining garlic seedlings by using intermittent immersion cultivation mode

A technology of submerged culture and garlic is applied in the field of rapidly obtaining garlic seedlings to achieve the effects of reducing mutation frequency, improving automation level and plant proliferation efficiency, and reducing consumption of manpower and material resources.

Inactive Publication Date: 2014-07-16
NANJING TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since this culture method better solves the problem of vitrification of plant tissue during liquid culture, and the nutrients can be well transferred with the immersion of plant tissue, so intermittent immersion culture can well solve the problem of plant tissue culture. various problems

Method used

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  • Method for quickly obtaining garlic seedlings by using intermittent immersion cultivation mode
  • Method for quickly obtaining garlic seedlings by using intermittent immersion cultivation mode
  • Method for quickly obtaining garlic seedlings by using intermittent immersion cultivation mode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The garlic bud explants were multiplied using a batch submerged culture reactor, and the specific steps were as follows:

[0040] (1) Proliferation culture: The specific method is to inoculate the sterilized garlic buds as explants into a pre-sterilized batch submerged culture reactor under aseptic conditions. The parameters of intermittent immersion culture were: immersion frequency: 10 min / 3h; inoculation density: 50 / L; culture time: 20 days. Proliferation medium formula is MS+2.0 mg / L 6-BA+0.1 mg / L NAA+2.0 mg / L KT+30 g / L sucrose, pH5.5. The culture conditions are: light intensity 1500-2000 lux, light 10 h / d, temperature 25±1°C. The growth status of garlic seedlings at the end of the multiplication culture in the batch submerged culture reactor was as follows: figure 1 shown.

[0041] (2) Microbulb generation culture: the specific method is to replace the proliferation culture medium with the bulb generation culture medium in the same reactor after the proliferati...

Embodiment 2

[0044] The clustered buds of garlic are propagated as explants in a batch submerged culture reactor, and the specific steps are as follows:

[0045] (1) Proliferation culture: The specific method is to inoculate the garlic cluster buds into a pre-sterilized batch submerged culture reactor under aseptic condition. The parameters of intermittent immersion culture were: immersion frequency: 8 min / 3h; inoculation density: 60 / L; culture time: 22 days. Proliferation medium formula is MS+2.0 mg / L 6-BA+0.1 mg / L NAA+2.0 mg / L KT+30 g / L sucrose, pH5.8. The culture conditions are: light intensity 1500-2000 lux, light 12 h / d, temperature 25±1°C.

[0046] (2) Rooting and microbulb generation: the specific method is to replace the proliferation culture medium with the bulb generation culture medium in the same reactor after the proliferation culture is over to continue the culture. The bulb generation culture solution is 1 / 2MS+1.0 mg / L IBA+0.01 mg / L NAA+5 mg / L paclobutrazol+45 g / L sucrose,...

Embodiment 3

[0049] The garlic flower buds were multiplied using a batch submerged culture reactor. The specific steps were as shown in Example 1, except that the formula for the selected proliferation culture medium was MS+4.0 mg / L 6-BA+0.2 mg / L NAA +2.0mg / L KT+30 g / L sucrose, pH5.8. The culture medium for bulb generation was 1 / 2MS+2.0 mg / L IBA+0.1 mg / L NAA+5 mg / L paclobutrazol+45 g / L sucrose, pH5.8. The proliferation efficiency is over 1:28 within one culture cycle. Type ① and type ② miniature bulbs account for 75%, type ③ miniature bulbs account for 25%, and the survival rate of the obtained tissue culture seedlings is over 90%.

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Abstract

The present invention relates to a method for rapidly obtaining garlic seedlings by means of intermittent immersion culture, in which buds of garlic clusters or sterilized flower buds are used as inoculation materials, and transferred to an intermittent immersion culture reactor for two stages of induction of proliferation and cultivation of bulbs. At the first stage, after the end of the multiplication culture, the multiplication culture medium was replaced by the bulb generation culture medium under aseptic conditions to promote the formation of small garlic bulbs, and transplanted to obtain a large number of uniform garlic seedlings. The method greatly improves the degree of automation in the production process of garlic seedlings, reduces manpower input during cultivation, and can greatly increase the multiplication rate. The use of the reactor to produce garlic seedlings has the advantages of no pathogenic bacteria, uniform and stable genetics, etc., and the survival rate is significantly improved, and the labor cost is significantly reduced. The method reduces the seedling cost while improving the quality of the garlic seedlings, and provides guarantee for the low-cost production of a large amount of high-quality garlic bulbs.

Description

technical field [0001] The invention relates to a method for rapidly obtaining garlic seedlings by means of intermittent immersion culture, and belongs to the technical field of plant cell engineering. In particular, it relates to a method for rapidly obtaining large batches of seedlings (bulbs) by utilizing intermittent immersion bioreactors for garlic tissue culture and large-scale rapid propagation. Background technique [0002] garlic( Pinellia spp.) is an indispensable condiment in people's daily life, and has always been loved by the masses of the people. And garlic has the effects of regulating immunity, protecting the liver, anti-oxidation, anti-mutagenesis and anti-cancer. [0003] The traditional garlic cultivation is to plant garlic cloves as planting materials. This method has two obvious disadvantages. One is that garlic cloves will consume a large amount of garlic products as planting materials, and its reproduction rate is low. Usually, one garlic clove c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈集双贾明良张本厚王菁菁丁爱军欧阳平凯
Owner NANJING TECH UNIV
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