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Tissue culture and rapid propagation method for pinellia tuber plant

A technology for tissue culture and rapid propagation of Pinellia, which is applied in the field of plant cell engineering, can solve problems such as increasing labor input, and achieve the effects of high automation level, large biological output, and reduced mutation frequency

Inactive Publication Date: 2013-05-15
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These have greatly increased the labor input in the tissue culture production process

Method used

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  • Tissue culture and rapid propagation method for pinellia tuber plant
  • Tissue culture and rapid propagation method for pinellia tuber plant
  • Tissue culture and rapid propagation method for pinellia tuber plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The specific steps for multiplying the petiole explants of the aseptic seedlings of Pinellia trifoliata by using a batch immersion submerged culture reactor are as follows:

[0045] (1) Selection of intermittent immersion culture reactors: use the patent No. ZL 200920116327.X of "Cultivation reactors for intermittent immersion of plant tissues and organs". The volume size is 3L.

[0046] (2) Material selection and treatment: The petiole of the sterile Pinellia trifoliate seedlings obtained by conventional tissue culture methods was used as explants, and the petiole that grew vigorously was taken, and the middle part of the petiole was cut into lengths of 0.5, 1, 1.5, and 2 cm, respectively. Several kinds, later used as explant material for inoculation in the next step.

[0047] (3) Proliferation culture: the specific method is to inoculate the treated explants into the pre-sterilized batch immersion culture reactor under aseptic condition. The parameters of the batch ...

Embodiment 2

[0052] Concrete steps for multiplying the clustered bud explants of Pinellia aseptic seedlings by using a batch submerged culture reactor:

[0053] (1) Selection of intermittent immersion culture reactors: use the patent No. ZL 200920116327.X of "Cultivation reactors for intermittent immersion of plant tissues and organs". The volume size is 5L.

[0054] (2) Material selection and processing: 2) Material selection and processing: The differentiated cluster buds of sterile Pinellia trifoliate tissue cultured seedlings obtained by conventional tissue culture methods are used as explants, and the cluster shoots with unexpanded leaves are taken in a good state , cut into a single state and used as explant material for inoculation.

[0055] (3) Proliferation culture: the specific method is to inoculate the treated explants into the pre-sterilized batch immersion culture reactor under aseptic condition. The parameters of the batch immersion culture reactor were set as follows: imm...

Embodiment 3

[0059] The specific steps for multiplying the leaf explants of the aseptic seedlings of Pinellia trifoliata by using a batch submerged culture reactor are as follows:

[0060] (1) Selection of intermittent immersion culture reactors: use the patent No. ZL 200920116327.X of "Cultivation reactors for intermittent immersion of plant tissues and organs". The volume size is 1L.

[0061] (2) Material selection and treatment: The leaves of sterile Pinellia tissue-cultured seedlings obtained by conventional tissue culture methods were used as explants, and the fully expanded leaves were taken, and three wounds were cut across them for the next inoculation explant material.

[0062](3) Proliferation culture: the specific method is to inoculate the treated explants into the pre-sterilized batch immersion culture reactor under aseptic condition. The parameters of the batch immersion culture reactor were set as follows: immersion frequency: 8-20 min / 2h; culture time: 15-30 d. The cultu...

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Abstract

The invention relates to a tissue culture and rapid propagation method for a pinellia tuber plant and belongs to the technical field of plant cell engineering. Organs such as sterile blades, leaf stalks, cluster buds and the like of pinellia tuber are used as inoculating materials. The method comprises the following steps of: transplanting the inoculating materials into an intermittent submerged culture reactor, and performing two stages of proliferating and inducing, and rooting and generating and culturing tubers; and after the proliferating culture is finished, replacing a proliferation culture medium with a rooting and tuber generating culture medium under the aseptic condition so as to promote the forming of the tubers of the pinellia tuber. By the method, the automation degree in the production process of pinellia tuber seedlings is greatly improved, the human input is reduced during the culture, and the proliferation rate is greatly improved. The pinellia tuber seedlings which are produced by the reactor have the advantages of no pathogenic bacteria, uniform and stable heredity and the like. The survival rate is obviously improved, the labor cost is obviously lowered, and the guarantee for producing a great number of high-quality seedlings and isolated tubers at low cost is provided. By the method, the seedling quality during the plantation of pinellia tuber medicinal materials is improved, the seedling cost is lowered, and economic benefits are obviously improved and the same time.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of pinellia plants, belonging to the technical field of plant cell engineering. Specifically, it relates to a method for obtaining large-scale seedlings (tubers) by using a high-throughput bioreactor for tissue culture and large-scale rapid propagation of Pinellia and its close species. Background technique [0002] Pinellia ( Pinellia spp.) is an araceae plant, and its tubers can be used as medicine. It has a medicinal history of more than 2,000 years and is an important Chinese medicinal material in my country. Wild pinellia medicinal materials are mainly distributed in Sichuan, Hubei and Henan provinces, followed by Jiangsu, Shandong, Jiangxi and Zhejiang provinces. According to statistics, among 558 kinds of traditional Chinese medicine prescriptions, Pinellia is the 22nd most frequently used medicinal material. Modern pharmacological studies have shown that Pinellia medi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈集双贾明良张本厚何珣刘学威
Owner NANJING TECH UNIV
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