PRP ribose extraction method

Abstract

The invention relates to a Haemophilus influenzae type B polyribosyl ribitol phosphate purification method, which comprises the following steps: 1) removing thallus in a Haemophilus influenzae type fermentation broth to obtain a fermented supernatant; and 2) removing nucleic acid and protein in the fermented supernatant to obtain PRP polysaccharide.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for preparing PRP ribose. Background technique [0002] Haemophilus influenzae type b (Hib) is the main pathogenic factor causing meningitis and otitis media in infants and young children. repeat unit. Studies have found that anti-PRP polysaccharide antibodies can effectively reduce Hib infection, so PRP-based polysaccharide vaccines or conjugate vaccines have become the main method to prevent Hib infection. [0003] Hib conjugate vaccine is the earliest and most successful conjugate vaccine developed in the world. The successful development of this type of conjugate vaccine has solved the shortcoming that polysaccharide vaccines cannot effectively stimulate the immune response of infants. With the development of conjugate vaccine technology, there are now many Pharmaceutical companies have developed different types of Hib conjugate vaccines; [0004] Haemophilus ...

AI Technical Summary

Problems solved by technology

[0006] The traditional PRP polysaccharide extraction method is cumbersome, the recovery rate is low, and a large amount of organic reagents such as phenol and acetone are used. These organic reagents have a potential impact on the environment; Sub-consistency puts forward higher requirements. Of course, the process of extracting protein with phenol is difficult to scale up and has the disadvantages of repeated operations.

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