CREB5 gene application

Abstract

The invention provides a CREB5 gene application, and relates to the preparation of products to distinguish latent tuberculosis infection and active tuberculosis. The preferred products include the products utilizing real-time quantitative PCR or gene chip detection to distinguish the latent tuberculosis infection and the active tuberculosis. According to the experimental results, the expression of the CREB5 gene is obviously higher in the blood of tuberculosis patients than in healthy people or latent infection crowds, and therefore, the CREB5 gene can serve as a special marking gene for the diagnosis of tuberculosis, so as to allow the tuberculosis diagnosis to be more accurate and quicker.

Description

Technical field: [0001] The invention relates to the technical field of bioengineering, in particular to the application of CREB5 gene. Background technique: [0002] Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis infection. Mycobacterium tuberculosis can not only cause pulmonary tuberculosis (85%), but also cause tuberculosis in multiple organs outside the lungs. Although there are currently effective anti-tuberculosis drugs, tuberculosis is still the number one killer of infectious diseases, and about 2 million people die from tuberculosis every year in the world; about one-third of the world's population is infected with Mycobacterium tuberculosis, which is called About 10% of people with latent tuberculosis infection (LTBI) will eventually develop active tuberculosis. [0003] Due to the lack of effective tuberculosis vaccine, the prevention and control of tuberculosis mainly depends on early detection, treatment and isolation of ...

AI Technical Summary

Problems solved by technology

However, current detection techniques for diagnosing active tuberculosis have serious deficiencies and cannot meet the requirements of clinical and tuberculosis prevention and control: 1) Sputum Mycobacterium tuberculosis microbiological examination is highly specific and is currently the gold standard for diagnosing active tuberculosis. Low reliability (less than 40%), long time-consuming (1-2 months for tuberculosis culture), and high requirements for laboratory biosafety

2) Mycobacterium tuberculosis gene detection, although the purpose of rapid diagnosis (1 day) has been achieved, the sensitivity of genetic detection directly from sputum samples has not been significantly improved, and there are problems of false negatives and false positives

3) Antibody detection in immunological testing is deemed unsuitable for the diagnosis of tuberculosis by the World Health Organization; cellular immunological testing includes tuberculin skin test (TST) and tuberculosis interferon release test (IGRA), which cannot effectively distinguish patients with active tuberculosis and latent tuberculosis infection, although the latter is significantly more sensitive than other tests in patients with active tuberculosis

[0004] CREB5 is also called CRE-BPA, CRE-BP alpha, cAMP response element binding protein, which can activate transcription. It contains a zinc finger and a leucine zipper DNA binding domain. Currently, there is little research on the function of this gene

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