Rapid identification method of tobacco black shank resistance

Abstract

The invention discloses a rapid identification method of tobacco black shank resistance. The rapid identification method of the tobacco black shank resistance includes the following steps of (1) sterilizing the surface of tobacco seeds to be identified; (2) cultivating tobacco blank shank germs and preparing suspension liquid of conidium of the tobacco blank shank germs; (3) extracting toxin liquid of the tobacco blank shank germs; (4) germinating the seeds after soaking seed in the toxin liquid and calculating rate of emergence of the seeds and evaluating the resistance. The rapid identification method of the tobacco black shank resistance is easy to operate, low in cost, rapid, high in accuracy, and appropriate for black shank resistance identification in tobacco breeding research.

Description

technical field [0001] The invention belongs to the technical field of crop disease resistance identification, and in particular relates to a rapid identification method for tobacco black shank resistance. Background technique [0002] Tobacco black shank (Phytophthora parasitica var.nicotiane) is one of the most destructive diseases in tobacco production. It is the most important factor restricting the production of tobacco leaves in various tobacco areas in my country. It can harm flue-cured tobacco, air-cured tobacco, sun-cured tobacco, and Burley tobacco. , oriental tobacco and all other cultivated tobaccos. Tobacco black shank was first discovered in Java by Bred de Haan in 1896. Cook observed the disease in Puerto Rico in 1924. Since then, tobacco black shank has been reported successively in major tobacco-producing areas in the world, and now the disease has spread in temperate, subtropical and tropical smoking areas. In the 1940s, the disease area was expanded to t...

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Problems solved by technology

Among them, the identification of disease resistance is mainly used in the identification of adult tobacco plants, and the identification results are easily affected by the uniformity of the colony concentration of the plot and the climate, resulting in certain differences in the identification results between different years, and the identification of disease areas is also difficult. Due to the limited area, it is impossible to carry out resistance identification on large quantities of materials at the same time; the identification of seedling inoculation overcomes the limitations of identification of disease nurseries to a certain extent, but in the application process due to the age of inoculation seedlings, inoculation methods, and strains of pathogens As well as different culture environments, the identification results are often inconsistent

The identification results of the above two methods only reflect the tolerance of tobacco seedlings or adult plants to black shank, but not the true resistance level of the variety or material, because: there are often Latent infection phenomenon, that is, black shank pathogen invades the plant through root wounds and continues to multiply and extend in the stem and other vascular bundles. Only when the number of colonies in the plant reaches a certain level, the plant will show wilting symptoms; at the same time, different resistance The ability of resistant materials to inhibit the proliferation and extension of black shank bacteria is also different, so it is impossible to truly evaluate the resistance level of different materials simply by judging by the symptoms of plants

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