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Method for extracting tripterine

A technology of tripterygne and its extract, which is applied in the field of extracting tripterygne, and can solve the problems of small preparation amount, complicated process and low yield

Inactive Publication Date: 2013-05-08
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the extraction and preparation of tripterine is currently limited to a small-scale laboratory. The past technology is often difficult to scale up because of low yield, high cost, small preparation amount or complicated process, resulting in low utilization rate of plant resources. Low, currently the price of Tripterygium wilfordii in the market is very expensive

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  • Method for extracting tripterine

Examples

Experimental program
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Effect test

Embodiment 1

[0042] This embodiment is used to illustrate the method for extracting tripterine provided by the present invention.

[0043] Get 218 grams of the dry root bark of S. chinensis of the genus S. chinensis and pulverize it. Each time, use 1200 mL of methanol to reflux extract for 1 hour, filter, and extract three times in total. The extraction products obtained each time are mixed to obtain 3390 mL of Tripterygium wilfordii. The content of element is the alcohol extract of 0.0625% by weight. The methanol in the ethanol extract is removed to obtain an extract. The extract was suspended in 300 mL of water and then extracted with 250 mL of chloroform each time for a total of 3 extractions. After the chloroform extract was concentrated to about 8mL (the content of tripterine in it was 1.98g), the sample was mixed with 20g of diatomaceous earth and dried. The product mixed with diatomaceous earth is placed in the upper layer of a silica gel column with 95 grams of 200-300 mesh silic...

Embodiment 2

[0045] This embodiment is used to illustrate the method for extracting tripterine provided by the present invention.

[0046] Take 1000 grams of the dried root bark of the plant of the genus S. hortifolia, crush it, extract it by percolation with ethanol at room temperature, and obtain 38 liters of ethanol extract with a tripterine content of 0.0169% by weight. The ethanol in the ethanol extract is removed to obtain an extract. The extract was suspended in 500 mL of water and then extracted with 300 mL of chloroform each time for a total of 5 extractions. Concentrate the chloroform extract to 18mL (the content of tripterine in it is 6.02g), mix the sample with 38g of diatomaceous earth, and dry it. Diatomite mixed sample product is placed in the upper layer of a silica gel column with 170 grams of 200-300 mesh silica gel inside for adsorption chromatography separation. After the low polar components are eluted with petroleum ether, petroleum ether and ethyl acetate are used t...

Embodiment 3

[0048] This embodiment is used to illustrate the method for extracting tripterine provided by the present invention.

[0049]Get the dry root bark of 150 grams of Tripterygium genus Tripterygium genus Tripterygium and pulverize, use 800mL methanol ultrasonic extraction each time, filter, extract altogether three times, wherein, the extraction time for the first time is 30 minutes, and the extraction time of last 3 times is After 20 minutes, the extracted products obtained each time were mixed to obtain 2210 mL of tripterine content of 0.0373 wt % alcohol extract. The methanol in the ethanol extract is removed to obtain an extract. The extract was suspended in 200 mL of water and then extracted with 200 mL of dichloroethane each time for a total of 7 extractions. Concentrate the dichloroethane extract to a small volume (about 3 mL, the content of tripterine in it is 0.82 g), mix the sample with 8 g of diatomaceous earth, and dry it. The product mixed with diatomaceous earth w...

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Abstract

The invention discloses a method for extracting tripterine. The method for extracting the tripterine comprises the following steps of: contacting a plant raw material containing the tripterine with low-level fatty alcohol, so that an alcohol extract with the tripterine content not less than 0.005wt% is obtained; then removing the low-level fatty alcohol in the alcohol extract, so as to obtain extract; then suspending the extract with water, then extracting with C1-C5 halogenated alkane, concentrating extract liquor and separating by adopting a silicagel column, collecting eluent containing the tripterine, and concentrating and crystallizing the eluent. By adopting the method for extracting tripterine disclosed by the invention, and the tripterine with high purity and yield can be obtained.

Description

technical field [0001] The invention relates to a method for extracting tripterine. Background technique [0002] Tripterine, also known as celastrol, celastrol, celastrol, or celastrol or tripterine in English, is a quinone methyl pentacyclic triterpenoid compound, and its structure is as shown in formula (I) Show. Tripterygium widely exists in the root bark of Euonymus plants Tripterygium genus and Serpentine genus. The molecular formula of tripterine is C 29 h 38 o 4 , red needle-like crystal, it is insoluble in water, soluble in organic solvents such as methanol, ethanol, acetone, ethyl acetate, chloroform, etc., the melting point is 185-200°C, and it has the strongest absorption at 425nm by ultraviolet full-wavelength scanning . [0003] [0004] Studies at home and abroad have found that tripterygium has a variety of medicinal effects, such as anti-oxidation, anti-inflammatory immunity, anti-tumor, anti-fertility, anti-fibrosis, anti-atherosclerosis, inhibitio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 王发松张升辉向家桂邓仕明张建林
Owner HUBEI UNIV FOR NATITIES
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