Composition containing salted fermented Korean ginseng extract
A technology of Korean ginseng and composition, applied in the direction of drug combination, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of functional component damage, corruption or pollution, etc., and achieve the effect of reducing the effect and excellent skin regeneration effect
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[0041] [Example 1-4] Production of Salted Fermented Red Ginseng Extract
[0042] Put 1 kg of Korean ginseng and an appropriate amount of boiling salt into a pottery so that the Korean ginseng is properly covered with boiled salt, and aged for 7 days. Then, after washing briefly, steam it in a high-pressure steam sterilizer at 98°C for 3 to 24 hours to make red ginseng. Furthermore, it dried for 24 hours with the hot-air drier of 60 degreeC. Then, 10% by weight of koji and 2% salt (boiled salt) water to the extent of covering the red ginseng were added to the weight of the dried red ginseng, and fermentation was carried out for 4 to 5 days. Then, after simple washing, 45 g of the salted red ginseng fermented extract was obtained using water or an organic solvent, which was used as Example 1.
[0043] Using substantially the same method as in Example 1, but using solar salt instead of boiled salt, 55 g of fermented salted red ginseng extract was obtained, which was used as Exa...
experiment example 1
[0046] [Experimental example 1] Evaluation of cell regeneration
[0047] In order to evaluate the cell regeneration effect of the salted fermented Korean ginseng extract, the following experiments were carried out. First, inoculate 1×10 in a 96-well plate 4 HaCaT cells / well. Replace with the medium without adding FBS, and incubate for 1 hour. Then, the medium was removed, and 1, 5, 10, 50, and 100 ppm of Example 1 and 10, 50, and 100 ppm of the salted and fermented Korean ginseng of Examples 2 to 4 were dissolved in a medium containing 1% FBS. After extraction, proceed to processing. At this time, the medium containing 1% and 10% FBS was used as a positive control group. After culturing for 48 hours, WST-1 was treated at 100 μL / well. Then, at 37°C, 5% CO 2 Incubate for 3 hours in the incubator. After culturing, the absorbance at 450 nm was measured with a microplate reader to confirm the cell regeneration effect. show the result in figure 1 .
[0048] Such as figure...
experiment example 2
[0049] [Experimental example 2] Cytotoxicity evaluation (MTT analysis)
[0050] In order to evaluate the cytotoxicity of the salted fermented Korean ginseng extract, the following experiments were carried out. First, inoculate 1×10 in a 96-well plate 4 HaCaT cells / well. After culturing for 24 hours, the cells were further cultured for 24 hours in a medium to which no FBS was added. Then, the culture medium was removed, 100 μl of PBS was added, and then 40 mJ of UVB was irradiated. After treating the salted fermented red ginseng extract of Example 1 at 1, 10 and 100 ppm and culturing for 48 hours, each was put into 100 μl of MTT solution (0.5 mg / mL), and further cultured in an incubator at 37° C. for 3 hours. At this time, the experiment was also performed on the control group which was not irradiated with UVB. The solution was removed, and the formed formazan was dissolved in 100 µl of DMSO, and the absorbance at 570 nm was measured to confirm cytotoxicity. show the resul...
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