Shellfish artificial ooze and preparation method thereof
An artificial and ooze technology, applied in the field of shellfish artificial ooze and its preparation, can solve the problems of inapplicable activity observation, etc., and achieve effective observation and recording with good results
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Embodiment 1
[0019] Screening of agar ratio
[0020] 1. Cut the agar strips into pieces with scissors, and use an electronic balance to weigh three portions of 0.1g, 0.2g, 0.4g, 0.6g and 0.8g of agar respectively. Take 15 250ml beakers, put the above-mentioned agar into the beakers respectively, and each gradient has three parallels. Add 200ml of seawater into each beaker, and heat and stir until the agar is completely dissolved.
[0021] 2. After the solution is cooled and solidified, put one cockle (weight 3.2136±0.173g) into each beaker, and observe its activities and burrowing conditions on agar with different hardness.
[0022] 3. Experimental results, as can be seen from Table 1 below, the activity behavior of 2-year-old cockles on agar surfaces with different hardnesses is different, and the concentration greater than 3 mg / ml or less than 1 mg / ml has an impact on the activities of cockles.
[0023] Table 1 The degree of softness and hardness of different concentrations of agar sol...
Embodiment 2
[0031] Choice of background color
[0032] 1. Select four kinds of pigments such as natural pigments (lemon yellow, lycopene, spinach pigment, gardenia blue pigment) and add them to the agar solidified body (concentration is 1-3 mg / ml) in Example 1 to make it appear yellow, Red, green, blue and other different colors, then add cockles to the beakers of each color, and observe the activities of the cockles. Within 24 hours, cockles only had actions (digging and shell opening and closing) in green (spinach pigment) and blue (using gardenia blue pigment) agar solidified solids, but basically had no burrowing activities in yellow and red agar , only the shell opening and closing action. It can be considered that red and yellow backgrounds are not suitable for cockles. Colors such as dark blue and dark green basically do not affect the experimental shellfish and daily activities.
[0033] 2. Set the addition amount of gardenia blue pigment to be 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, ...
Embodiment 3
[0036] Selection of Antibiotics in Artificial Slime
[0037] In the above-mentioned 2-week test, it was found that some beakers had plaques, etc., and it was necessary to add antibiotics to inhibit the bacteria, so as to ensure that the artificial slime would not grow plaques or deteriorate during use.
[0038] Choose conventional antibacterial, antiactinomycetal, and antifungal antibiotics. Taking into account the presence of overlying water and shellfish activities may result in the loss of a certain amount of antibiotics into the overlying water. Therefore, different amounts of antibiotics were used for experiments at different times. For short-term (5-7 days) tests such as behavioral observation, low-concentration formulations can be used (Table 3, Table 4), and for long-term (30-day) tests such as growth, activity, and feeding, high-concentration formulations can be used (Table 3 , Table 4), the solvent used in the following formulations is distilled water.
[0039] Lo...
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