Camptotheca endophytic bacterium LY214 for producing camptothecin and application thereof

Abstract

The invention relates to a camptotheca endophytic bacterium which is obtained from camptotheca acuminate through separation and purification, and an application of the camptotheca endophytic bacterium. The camptotheca endophytic bacterium LY214 involved by the invention is identified to be paenibacillus polymyxa by morphology and molecular biology; and the bacterial strain is preserved in the China General Microbiological Culture Collection Center, the preservation date is November 19th, 2012, and the preservation number is CGMCC 6841. The camptotheca endophytic bacterium is characterized in that the high performance liquid chromatography, the ultraviolet spectrum and the mass spectrometry show that the camptotheca endophytic bacterium can generate an antineoplastic active component which is camptothecin through liquid fermentation. The invention discloses for the first time that the endophytic bacterium for producing camptothecin can be separated and cultured from the camptotheca acuminate, and provides a new way for the preparation of camptothecin.

Description

technical field [0001] The invention relates to the isolation, cultivation and application of a camphor tree endophytic bacterium, belonging to the field of medical biotechnology. Background technique [0002] Camptothecin (CPT) is a terpenoid indole alkaloid formed by the condensation of tryptamine and split loganin. It was first isolated from the bark of the unique plant Camptotheca acuminata in my country, and was later found in many plants. Such as Ervatamia heyneana of Apocynaceae, Nothapodytes foetida of Tea Cornaceae, and Ophiorrhiza pumila of Rubiaceae. Camptothecin has a unique anti-tumor mechanism, and exerts anti-tumor activity by inhibiting topoisomerase I. Camptothecin series derivatives are effective against pancreatic cancer, prostate cancer, breast cancer, lung cancer, ovarian cancer, colon cancer, cervical cancer, gastric cancer and blood tumors, and are the main varieties in the global anti-tumor drug market. [0003] The main sources of camptothecin are 1...

AI Technical Summary

Problems solved by technology

In addition, the use of a large amount of organic solvents in the extraction process leads to large environmental pollution and cumbersome steps

The chemical total synthesis of camptothecin is synthesized by different ring-building methods, such as split method, asymmetric oxidation method, etc. Most methods have shortcomings such as too long routes and low total yields, making it difficult to realize industrialization

The use of plant cell culture to produce high value-added plant secondary metabolites (such as camptothecin and its derivatives) was once considered a promising alternative, but due to the slow growth of plant cells and the heterogeneity of the culture process Factors such as severe globalization, unstable output, and production costs make it difficult to realize industrialized production

In short, the sources of camptothecin are limited and cannot meet the market demand at all (the annual demand for camptothecin in the international market is 3000kg, but the global annual output of camptothecin is only 600kg), and it is necessary to discover and develop alternative resources and technologies

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