Reagent for assisting identification of arabis mosaic virus and application thereof

An auxiliary identification and leaf virus technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, fluorescence/phosphorescence, etc., can solve the problems of complicated ELISA technology operation steps, expensive electron microscope technology equipment, prone to false positives, etc. Broad application range and prospects, avoid false positive results, reduce the effect of false positive results
CN103173576AInactive Publication Date: 2013-06-26粟智平
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
粟智平
Publication Date
2013-06-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention aims at providing a reagent for assisting the identification of arabis mosaic virus and an application thereof. The reagent provided by the invention comprises a specific primer consisting of DNA (Deoxyribonucleic Acid) shown in a sequence 1 in a sequence table, DNA shown in a sequence 2 in the sequence table, and DNA shown in a sequence 3 in the sequence table. The reagent also comprises a specific probe shown in a sequence 4 in a nucleotide sequence table. The arabis mosaic virus is an important quarantine pest in China, and a method for detecting ArMV by semi-nested-RT (Reverse Transcriptase)-Realtime PCR (Polymerase Chain Reaction) is established by utilizing three nested type PCR primers and a TaqMan Probe. According to the method, the nested PCR technology and the real-time fluorescence PCR technology are organically combined; two PCR systems formed by the three primers and the probe are verified with each other, so that the result accuracy is effectively improved; the detection flexibility is effectively improved by the real-time fluorescence PCR technology; and the accuracy, the flexibility, the simplicity, the convenience and the rapidness are realized, and the minimum detection limit can reach 0.5fg / mul plant total RNA (Ribonucleic Acid).
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Description

technical field

[0001] The invention relates to a reagent for assisting identification of Arabidopsis mosaic virus and its application. Background technique

[0002] Arabis mosaic virus (ArMV) is a member of the genus Nepovirus of the family Comoviridae. Its geographical distribution is extremely wide, occurring in more than 30 countries on five continents, and it is a worldwide disease. There is no report on the harm of this virus in our country, and it is an important quarantine plant virus in my country.

[0003] The virus has a wide host range and can infect about 174 genera and 215 species of plants. It mainly harms economic crops such as cucumbers, lettuce, potatoes, carrots, tulips, tobacco, cherries, grapes, strawberries, spinach, roses, soybeans, and kidney beans.

[0004] ArMV can be transmitted over long distances through vegetative propagation materials such as seeds, scales, tubers and seedlings. In the field juice, grafting, etc. can spread the virus. Seeds...

Claims

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