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Automatic device and methods for particle analysis

An automatic device and particle technology, applied in individual particle analysis, particle and sedimentation analysis, measuring devices, etc., can solve problems such as labor and time consumption

Inactive Publication Date: 2013-06-26
陶靖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the consumption of manpower and time to the pretreatment of sample in the flow cytometric analysis, and the problem that additional manual or automated analyzer is needed to detect the absolute concentration (number / liter) of the cells to be tested, the present invention A device and method for microparticle analysis is proposed, which can automatically perform sample pretreatment and analysis, and can adaptively determine the optimal ratio of markers and substances to be labeled

Method used

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  • Automatic device and methods for particle analysis
  • Automatic device and methods for particle analysis
  • Automatic device and methods for particle analysis

Examples

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example 1

[0090] To find the concentration of lymphocytes in a whole blood sample, that is, lymphocytes need to be classified and absolutely counted, but not labeled.

[0091] Using the above-mentioned working mode A, the sample is directly added to the hemolysis and cell classification reaction pool 11 by the sample suction needle device 44 to react with the reagent, dissolve the red blood cells, and perform a certain treatment on the white blood cells. Then it is sent to the flow cytometry analysis system, combined with certain optical methods, to obtain the information of the absolute count of white blood cells and the proportion of lymphocytes to the whole white blood cells. Since the amount of samples and reagents participating in the reaction and the amount of each analysis are accurately measured, the device and method can further calculate the concentration of lymphocytes (number / liter).

example 2

[0093] To find the concentration of Helper subtypes of T lymphocytes in a large number of human whole blood samples, that is, to mark the Helper subtypes with CD3 and CD4 monoclonal antibodies, to obtain the proportion of Helper subtypes in lymphocytes; and to obtain The concentration of lymphocytes.

[0094] Using the above working mode B-1, follow the steps below:

[0095] a. Before the analysis test, the operator puts the required markers CD3 and CD4 monoclonal antibodies into two standard microcentrifuge tubes respectively, and then puts the standard microcentrifuge tubes into the cavities of the marker storage pool 22 respectively, and fix;

[0096] b. The sample is put into the hemolysis and cell classification reaction pool 11 by the sample suction needle device 44 to react with the reagent, and then sent to the flow cytometry analysis system for detection to obtain the absolute count of white blood cells and the ratio of lymphocytes to the whole white blood cells. Si...

example 3

[0101] To find the concentration of the Suppressor subclass of T lymphocytes in a small batch of human whole blood samples, that is, to mark the Suppressor subclass with CD3 and CD8 monoclonal antibodies, to obtain the ratio of the Suppressor subclass to lymphocytes; and to obtain The concentration of lymphocytes.

[0102] Using the above working mode B-2, proceed as follows:

[0103] a. The sample is put into the hemolysis and cell classification reaction pool 11 by the sample aspirating needle device 44 to react with the reagent, and then sent to the flow cytometry analysis system for detection to obtain the absolute count of white blood cells and the ratio of lymphocytes to the whole white blood cells. Since the amount of samples and reagents participating in the reaction are all accurately measured, the device and method can further obtain the concentration (number / liter) of white blood cells and lymphocytes;

[0104] b. The operator puts the required marker CD3 and CD8 m...

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Abstract

The invention discloses automatic device and methods for particle analysis. The device comprises a flow cytometry analysis system which can obtain absolute concentration (quantity / liter) of a target object to be tested. The device also comprises a sample pre-treatment system and comprises hemolysis and cell classification reaction pool, a marker storage pool, a marker reaction pool, a sample drawing needle device, a marker drawing needle device and a sample sampling position. The invention also discloses various methods for analyzing the target object to be tested by the device and concrete operation steps. By utilizing the device and the methods disclosed by the invention, automatic sample treatment and analysis can be carried out; an artificial sample pre-treatment process is removed; meanwhile, the quantity of the added markers can be adaptively adjusted aiming at the sample to be marked; and the optimal reaction ratio of the marker and the object to be marked is adaptively determined in the device; and the analysis accuracy and rapidity are improved.

Description

1. Technical field: [0001] The present invention relates to devices and methods for particle analysis. In particular, the present invention relates to an automatic device and method for microparticle analysis using flow cytometry analysis technology. 2. Background technology: [0002] Flow cytometry technology (Flow Cytometry) applies the principle of hydrodynamic focusing, so that the particles to be analyzed are arranged in a line and flow through the focused light detection area one by one. The light scattered by the particles and the excited fluorescence are captured by multiple Detector detection, through signal processing and information processing, realizes the classification and counting of targets. [0003] Flow cytometry analysis technology is widely used in the analysis of various particles. Here, microparticles refer to various cells of humans or other animals and plants and various artificial microspheres, such as microspheres made of organic materials or inor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10
Inventor 陶靖
Owner 陶靖
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