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Atherosclerosis targeting aptamer as well as preparation method and application thereof

An atherosclerosis and aptamer technology, applied in biochemical equipment and methods, preparations for in vivo tests, pharmaceutical formulations, etc., can solve problems such as difficulty in meeting clinical diagnostic requirements, and achieve early specific diagnosis, The effect of increasing target/non-target ratio and high signal-to-noise ratio

Active Publication Date: 2013-09-11
广州鹏兴医药科技有限公司
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  • Abstract
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Problems solved by technology

[0009]However, due to no targeting guidance, after USPIO is injected into the body, most of them are taken up by the reticuloendothelial system (RES), and have a certain targeting effect on AS vessels But it is difficult to meet the requirements of clinical diagnosis

Method used

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  • Atherosclerosis targeting aptamer as well as preparation method and application thereof
  • Atherosclerosis targeting aptamer as well as preparation method and application thereof
  • Atherosclerosis targeting aptamer as well as preparation method and application thereof

Examples

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Embodiment 1

[0064] A targeting aptamer for atherosclerosis, the preparation method of which is as follows:

[0065] 1) Culture smooth muscle cells: SD rats, male or female, weighing 150-200 g, 4-8 weeks old, after intraperitoneal anesthesia, aseptically remove the thoracic aortic segment, put it in a plate containing PBS solution and rinse 3 times After washing the clot, peel off the fibrous fat layer of the adventitia, then cut the blood vessel longitudinally, scrape off the intima, that is, the endothelial cells, quickly tear off the inner and middle layers of the media, cut into strips about 1mm wide, and soak them in water containing Serum in PBS solution, and the cut small tissue pieces were planted on the wall of the culture bottle, placed in a 37°C incubator for about 2 hours, took out the culture bottle and added 20% fetal bovine serum culture solution, and then put it back into the incubator to rest Cultivate for 4 days, after the cells cover the bottom of the bottle, digest and ...

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Abstract

The invention discloses an atherosclerosis targeting aptamer as well as a preparation method and an application thereof. The targeting aptamer is a targeting aptamer fragment for atherosclerosis, which is obtained by adopting an SELEX method, screening using macrophage-derived foam cells and also reversely selecting using smooth muscle cells, endothelial cells and THP-1 cells; by applying the targeting aptamer to preparation of an MRI (magnetic resonance imaging) atherosclerosis targeting nano-contrast agent, the targeting aptamer is only specifically combined with the macrophage-derived foam cells and combined with an AS (atherosclerosis) lesion vessel tissue with high specificity, so that the targeting property of the MRI atherosclerosis targeting nano-contrast agent is improved, and the early specific diagnosis of the atherosclerosis can be realized.

Description

[0001] Technical field [0002] The present invention involves magnetic resonance diagnostic reagents, which specifically involves a targeted matching and preparation method and application of an atherosclerosis. [0003] Background technique [0004] According to the World Health Organization (WHO), the number of deaths caused by atherosclerosis (AS) accounted for 52%of the number of deaths of all diseases, far exceeding the tumor (24%), which ranked second.The first killer of human health.AS mainly accumulates large and medium -sized muscle bullet artery, which shows the thickening of the arterial wall, and the narrowing of the lumen is the main basis for the diagnosis of clinical imaging.However, research in recent years has shown that the occurrence of clinical events caused by AS, such as acute coronary syndrome, often has no obvious correlation with arterial stenosis caused by AS.The occurrence and development of AS is a chronic and gradual process. Typical AS lesions can b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/115C12N15/10C12Q1/68A61K49/06A61K49/10
CPCA61K49/1848A61K49/1851A61K49/1857C12N15/115C12N2310/16G01R33/5601A61K49/085
Inventor 严鹏科
Owner 广州鹏兴医药科技有限公司
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