Extraction and perfusion culture system for MSCs (Mesenchymal Stem Cells)

Abstract

The invention discloses an extraction and perfusion culture system for MSCs (Mesenchymal Stem Cells), belongs to a field of medical instruments, and more specifically, provides a set of continuous and completed system design for obtaining high-purity stem cell products from a placenta organ. The device provided by the invention comprises an extraction device and a perfusion culture system, and the extraction device and the perfusion culture system are connected hermetically and aseptically, wherein the extraction device comprises a placenta cutting device, a reaction tank, a filter device, a filtrate collection device and a density gradient centrifugation device; and the perfusion culture system comprises a culture solution storer, a monitoring reactor, a cell culture bottle, a liquid waste bottle, a bottle for obtaining liquid, a biochemical criterion detection head group, an online monitoring system, a speed control fluid propeller and a pipeline device capable of controlling speed. The extraction and perfusion culture system for the MSCs (Mesenchymal Stem Cells) has benefits that the cutting efficiency is high; the operation is convenient and effective; the production efficiency can be greatly improved; the preparation technology is simple; and the quality control is simple and convenient, so as to efficiently obtain uniform and good stem cell products within a short time.

Description

technical field [0001] The invention relates to a medical mechanical device, in particular to a mesenchymal stem cell extraction perfusion culture system. Background technique [0002] Stem cells refer to primitive cells with self-renewal and multi-directional differentiation potential, which are the origin cells of the body, and can differentiate into one or more cells that constitute human tissues or organs under specific conditions. Mesenchymal Stem Cell (MSC) is a kind of stem cell. It is named for its ability to differentiate into mesenchymal tissue. It has subpotential differentiation potential and can be induced to differentiate into various tissue cells under specific in vivo and in vitro environments. . [0003] Placental mesenchymal stem cells refer to the stem cells that exist in the placenta after the newborn is born, which contain stem cells that match the fetal tissue and also contain stem cells that match the mother's tissue. In vitro induction and different...

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to disclose a stem cell extracting perfusion culture device to overcome the shortcomings of the existing manual cutting of the placenta to extract stem cells and static perfusion or only dynamic perfusion. More practical, more economical, more environmentally friendly, and more widely used systems and applications for extracting stem cells from placenta and perfusion culture in medical institutions and cell preparation application industries

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