Time resolution immunofluorescence analyzing method and kit for saccharides antigen 19-9

A carbohydrate antigen and time-resolved technology, which is applied to the analysis of materials, measuring devices, instruments, etc., can solve the problems of insufficient accuracy and achieve the effects of small cross-reaction, short detection time and high analytical sensitivity
CN103308683AActive Publication Date: 2013-09-18瑞孚迪生物医学(上海)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
瑞孚迪生物医学(上海)有限公司
Publication Date
2013-09-18

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Abstract

The invention discloses a time resolution immunofluorescence analyzing method and a kit for saccharides antigen 19-9. The analyzing method comprises the following steps of: 1, preparing a solid-phase antibody; 2, preparing a lanthanide ion labeled antibody; and 3, adding a standard product or a sample to be measured of the saccharides antigen 19-9 into a solid-phase material with a solid-phase antibody prepared in the step 1, performing reaction on a buffer liquid, and after the solid-phase material is incubated and washed, adding the diluted labeled antibody and incubating again to perform fluorescence detection. The invention also provides the corresponding kit. The method and the kit have the characteristics of high sensitivity, high specificity and high stability, short detection time and high coincidence rate of detecting national quality control products of CA19-9.
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Description

technical field

[0001] The present invention relates to a time-resolved immunofluorescence analysis method and a kit, in particular to a time-resolved immunofluorescence analysis method and a kit for carbohydrate antigen 19-9, and involves the use of mixed labeled antibodies to resolve the carbohydrate antigen 19-9 ( CA19-9) The compliance rate of national quality control products. Background technique

[0002] CA19-9 is a digestive tract tumor-associated carbohydrate antigen, secreted by gastrointestinal epithelial mucosal cells, with a molecular weight of about 5000KD, and its structure is a combination of Lea blood group antigen and sialic acid Lexa. High diagnostic value, especially for pancreatic cancer, gallbladder bile duct cancer and liver cancer; the positive rate of pancreatic cancer can be as high as 85%-95%. CA19-9 is often used for follow-up monitoring after treatment. When CA19-9 continues to increase, it indicates that there may be residual tumor or metastasi...

Claims

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