Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Time resolution immunofluorescence analyzing method and kit for saccharides antigen 19-9

A carbohydrate antigen and time-resolved technology, which is applied to the analysis of materials, measuring devices, instruments, etc., can solve the problems of insufficient accuracy and achieve the effects of small cross-reaction, short detection time and high analytical sensitivity

Active Publication Date: 2013-09-18
瑞孚迪生物医学(上海)有限公司
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the method of the present invention, the technical problem in the prior art about the insufficient accuracy of CA 19-9 detection of national quality control serum can be solved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Time resolution immunofluorescence analyzing method and kit for saccharides antigen 19-9
  • Time resolution immunofluorescence analyzing method and kit for saccharides antigen 19-9
  • Time resolution immunofluorescence analyzing method and kit for saccharides antigen 19-9

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] Example: Determination of Carbohydrate Antigen 19-9 (CA 19-9)

[0039] 1. Preparation of standard products

[0040] With 20% (v / v) calf serum, 0.1% NaN 3 (w / v) normal saline was prepared from carbohydrate antigen 19-9 purchased from Hytest Company into 0, 15, 50, 100, 200, 500 U / ml standard solutions. Aliquot and store at 2-8°C.

[0041] 2. Preparation of coated microporous reaction strips

[0042](1) Dilute the purified anti-carbohydrate antigen 19-9 monoclonal antibody (purchased from Hystest Company) to 1-10 μg / ml with 50 mM carbonate buffer solution of pH 9.3-9.7 (in this embodiment, 4 μg / ml was used) / ml), and then add 100 μl / well into each well of the microwell reaction strip, and let it stand for 5 to 24 hours;

[0043] (2) After washing the antibody-coated microwell reaction strip prepared in step (1) with a buffer solution containing a surfactant, add a buffer solution containing blocking protein, and let stand for 3 to 12 hours to block;

[0044] Wherein,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a time resolution immunofluorescence analyzing method and a kit for saccharides antigen 19-9. The analyzing method comprises the following steps of: 1, preparing a solid-phase antibody; 2, preparing a lanthanide ion labeled antibody; and 3, adding a standard product or a sample to be measured of the saccharides antigen 19-9 into a solid-phase material with a solid-phase antibody prepared in the step 1, performing reaction on a buffer liquid, and after the solid-phase material is incubated and washed, adding the diluted labeled antibody and incubating again to perform fluorescence detection. The invention also provides the corresponding kit. The method and the kit have the characteristics of high sensitivity, high specificity and high stability, short detection time and high coincidence rate of detecting national quality control products of CA19-9.

Description

technical field [0001] The present invention relates to a time-resolved immunofluorescence analysis method and a kit, in particular to a time-resolved immunofluorescence analysis method and a kit for carbohydrate antigen 19-9, and involves the use of mixed labeled antibodies to resolve the carbohydrate antigen 19-9 ( CA19-9) The compliance rate of national quality control products. Background technique [0002] CA19-9 is a digestive tract tumor-associated carbohydrate antigen, secreted by gastrointestinal epithelial mucosal cells, with a molecular weight of about 5000KD, and its structure is a combination of Lea blood group antigen and sialic acid Lexa. High diagnostic value, especially for pancreatic cancer, gallbladder bile duct cancer and liver cancer; the positive rate of pancreatic cancer can be as high as 85%-95%. CA19-9 is often used for follow-up monitoring after treatment. When CA19-9 continues to increase, it indicates that there may be residual tumor or metastasi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 于春红夏秋兰
Owner 瑞孚迪生物医学(上海)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com