Application of secretory protein 1 rich in cysteine in medical apparatus

A medical device and protein secretion technology, applied in the field of protein, to achieve good stability, intuitive and reliable result judgment, and convenient self-monitoring of the disease

Inactive Publication Date: 2013-09-18
苏州市马尔泰新材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on medical devices for early diagnosis of tongue cancer

Method used

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  • Application of secretory protein 1 rich in cysteine in medical apparatus
  • Application of secretory protein 1 rich in cysteine in medical apparatus
  • Application of secretory protein 1 rich in cysteine in medical apparatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Immunohistochemistry

[0018] Experimental materials: normal tongue tissue, tongue cancer tissue; the primary antibody is goat anti-human CRISP1 antibody, and the secondary antibody is donkey anti-goat Dylight594 antibody.

[0019] experimental method:

[0020] Tissue chip is the orderly combination of a large number of tissue samples on the surface of a micro-substrate, with the help of immunohistochemical method for detection.

[0021] 1. Fix the tissue with PFA fixative for 5 minutes, wash with PBS for 10 minutes

[0022] 2. Antigen retrieval: expose the epitope with 0.01M citrate solution. Microwave on high heat for 3 minutes to heat the repair solution to boiling (4 minutes), then continue to microwave on low heat for 1 minute, twice. Cool to room temperature and wash with PBS for 10 minutes. Membranes were ruptured with 0.5% Triton for 10 minutes.

[0023] 3. Block non-specific proteins

[0024] 1) Soak in PBS for 3 minutes x 3 times

[0025] 2) 3...

Embodiment 2

[0037] Example 2 Enzyme-linked immunosorbent assay (Elisa)

[0038] A total of 90 cases, including 30 cases of tongue cancer (squamous cell carcinoma) case group; 30 cases of other oral diseases (periodontal disease, pulp disease, oral candidiasis) group; 30 cases of healthy control group. The cysteine-rich secreted protein detection kit is used for detection, and the detection steps refer to the instruction manual of the cysteine-rich secreted protein quantitative enzyme-linked detection kit.

[0039] ELISA detection results of CRISP1:

[0040] 1. The tongue cancer group was higher than the other two groups, but there was no difference between the other disease group and the healthy group (P=0.802).

[0041] Table 1 The diagnostic value of salivary CRISP1 in the diagnosis of tongue cancer

[0042] diagnostic index AUC sensitivity specificity false positive rate false negative rate Youden Index Urine CRISP1 10 ng / ml 0.809 88.1% 39.3% 60.7% 11....

Embodiment 3

[0045] Example 3 Preparation of gold-labeled antibody complex on test paper

[0046] 1. Colloidal gold preparation

[0047] Add 100ml of 0.005%-0.02% chloroauric acid solution into a round bottom flask and heat to boiling. After boiling, add freshly prepared 0.4%-2% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) 1-2.2ml of aqueous solution, after boiling for 10-25 minutes, continue to stir and cool to room temperature. During this process, it can be seen that the color change of the solution is: golden yellow→black→purple→dark blue→bright red. When the color of the solution completely changes to transparent bright red, the required colloidal gold is obtained. After cooling, put it into a dialysis bag and dialyze it against ultrapure water (1:5000) three times, and finally transfer the dialyzed colloidal gold to a clean glass bottle with a screw cap, and store it in a dark environment at 4°C.

[0048] 2. Connection between gold colloid and protein

[0049] 1. The adsorption...

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Abstract

The invention relates to an application of a secretory protein 1 rich in cysteine in preparation of a medical apparatus for diagnosing tongue cancer. The medical apparatus is diagnostic test paper or a kit. The diagnostic sample of the medical apparatus is saliva. The invention has the advantages that a novel use of the secretory protein 1 rich in cysteine is provided, and a convenient, reliable, noninvasive and cheap method for screening crowds in a large scale with tongue cancer and self-detection of postoperative patients with tongue cancer is provided for guiding treatment of tongue cancer. The test paper provided by the invention is fast in detection, high in sensitivity, strong in specificity, good in stability, simpleness and convenience in operation, and no any instruments and devices. The result is intuitive and reliable to judge and easy to master, and the patient can conveniently monitor the own condition.

Description

technical field [0001] The present invention relates to a protein, in particular, the application of cysteine-rich secretory protein 1 in medical equipment. Background technique [0002] Tongue cancer can be manifested in three types in the early stage: ulcer, exogenous and infiltrating. In some cases, the first symptom is only tongue pain, which can sometimes be reflected to the temple or ear. The exophytic type can arise from malignant transformation of papilloma. The infiltrative surface may have no protrusions or ulcers. Ulcerative and infiltrating cancers are often accompanied by spontaneous pain and tongue movement limitation of varying degrees; exogenous types generally have no obvious tongue movement disturbance and less spontaneous pain. Advanced tongue cancer may directly exceed the midline or invade the floor of the mouth, and may also infiltrate the lingual periosteum, bone plate or bone of the mandible. Later, it can extend to the base of the tongue or the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 朱进安
Owner 苏州市马尔泰新材料有限公司
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