Quasi-automatic in-vitro detecting equipment used for detecting specific protein by turbidimetry and method thereof

Abstract

The invention discloses quasi-automatic in-vitro detecting equipment used for detecting a specific protein by turbidimetry and a method thereof. The equipment comprises a detecting bench; the detecting bench is provided with a photoelectric detection module with temperature control and stirring functions, a mechanical arm, a first loading pump, a second loading pump, a first container used for accommodating a first detecting reagent, and a second container used for accommodating a second detecting reagent; a reaction vessel used for pre-loading the first detecting reagent is arranged on the photoelectric detection module; the mechanical arm is provided with a first sampling needle used for loading the first detecting reagent and a second sampling needle used for loading the second detecting reagent. Automatic loading of the first detecting reagent and the second detecting reagent is realized by using the equipment, so that influence to the detecting results caused by volatilization of the first detecting reagent is avoided, labor cost of the specific protein detection is saved, artificial monitoring is unnecessary, and influence to the detecting results caused by the mistakes made in manual adding process of the detecting reagents are avoided as far as possible.

Description

technical field [0001] The invention relates to in-vitro diagnostic equipment, in particular to a quasi-automatic in-vitro detection equipment and method for measuring specific proteins by turbidimetry. Background technique [0002] The so-called specific proteins refer to those special proteins that have certain physiological functions in the body and play important pathological significance in disease states. Detection of these specific proteins can often determine the physiological condition of a patient's body. Existing specific proteins can be used for clinical detection of the following: immune dysfunction (detection of IgA, IgG, IgM, complement C3, complement C4, kappa light chain, lambda light chain); inflammation status monitoring (detection of C-reactive protein, alpha acid sugar protein, haptoglobin, ceruloplasmin); rheumatoid disease (detection of rheumatoid factor RF, antistreptolysin O, C-reactive protein) cardiovascular function monitoring (detection of high-...

AI Technical Summary

Problems solved by technology

[0006] 1) The detection reagent R2 needs to be manually added by the detection personnel. Since the reaction cup has a tapered opening at the upper end and a vertical narrow-diameter cavity at the lower end, when manually dropping the detection reagent, it is very easy to drop part of the detection reagent on the tester due to operational errors. On the inner wall of the reaction cup, the amount of the detection reagent participating in the reaction is insufficient, thereby affecting the detection result;

[0007] 2) The amount of detection reagent R2 added is generally a few microliters, which requires a high level of skill for the operator. A little carelessness will result in insufficient amount of detection reagent added, which is prone to errors, thus affecting the accuracy of the measurement results

[0008] 3) It takes a period of time for the reaction between the detection reagent R1 and the sample to be detected. When the instrument prompts the operator to add the detection reagent R2, because each operator's operating proficiency or the speed of adding the detection reagent will vary, so this will make the The reaction time between the detection reagent and the sample to be tested is different, resulting in differences between the test results; at the same time, this manual addition method increases the workload of the test personnel, and is prone to monitoring errors that affect the test results

[0009] 4) The detection reagent R1 is usually pre-installed in the reaction cup. If it is not used for a long time, it will volatilize to a certain extent, which will reduce the active ingredients of the detection reagent R1, so as to affect the final detection result.

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