Construction of cucumis interspecific introgression line populations, and method for applying same in cucumber breeding

Abstract

The invention relates to a construction of cucumis interspecific introgression line populations, and a method for applying the same in cucumber breeding, and belongs to the breeding field of biotechnology. A first interspecific introgression line populations of the cucumis is constructed by interspecific cross-backcross-self-cross of wild sour cucumber and cucumber and SSR molecular marker-assisted selection, wherein the interspecific introgression line populations are composed of 86 strains. The introgression line populations comprise introgressed segments of the wild sour cucumber covering 54% of whole genome of the cucumber; and three resources "IL2", "IL24" and "IL49" with relatively strong disease resistance are screened out through inoculation in field; the three resources are crossed with excellent cultivated cucumber respectively to obtain a series of cucumber novel spices with relatively strong disease resistance. The cucumber / sour cucumber interspecific introgression line populations not only broadens hereditary basis of the cucumber but also provides novel genetic resources for development and utilization of excellent genes of the wild sour cucumber, and lays a solid foundation for positioning important genes or genetic loci and functional study of the cucumber.

Description

technical field [0001] The invention relates to a method for constructing an interspecific introgression line population of the melon genus, and belongs to the field of biotechnology breeding. Background technique [0002] Cucumber is an important worldwide vegetable crop of the genus Cucurbitaceae. It is rich in nutrients, has many wild germplasm resources, and has many diseases and insect pests. However, the genetic basis of cucumber species is narrow, and the polymorphism is only 3%-8%. The long-term use of core germplasm resources has resulted in more and more single genetic backgrounds, fewer and fewer variant types of offspring, and resistance to various diseases and insect pests and natural adversity. capacity is also decreasing. Therefore, in the face of the depletion of excellent resources in the species and the bottleneck that is difficult to break through in conventional breeding, it is an important way to accelerate the process of cucumber breeding by digging ou...

AI Technical Summary

Problems solved by technology

However, in these studies, the screening of introgression lines was limited to the early backcross and self-crossing populations. There were many fragments introduced into a single plant, and fewer markers were used. The population coverage rate was low, and no screening of disease-resistant lines was carried out.

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