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Method for detecting polyether antibiotics in feed

A technology of polyether antibiotics and detection methods, which is applied in measurement devices, material separation, and analysis of materials, etc., can solve the problems of inability to detect, cumbersome steps, and increased detection workload, and achieves time saving, high sensitivity, and detection results. Accurate and reliable results

Inactive Publication Date: 2013-11-13
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Claims
  • Application Information

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Problems solved by technology

However, lasalocid sodium will decompose in this reaction environment and cannot react to obtain an ideal chromophoric group, resulting in inability to detect
At present, there is no relevant literature report on the simultaneous detection of polyether antibiotics lasalocid sodium, monensin, salinomycin and methyl salinomycin in feed samples at one time. In practice, these four polyether antibiotics Different methods are used to detect samples separately, which is cumbersome and increases the workload of detection

Method used

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  • Method for detecting polyether antibiotics in feed
  • Method for detecting polyether antibiotics in feed
  • Method for detecting polyether antibiotics in feed

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1 The establishment of standard working curve

[0025] (1) Preparation of reaction reagents

[0026] Preparation of monensin standard stock solution: Accurately weigh a certain amount of monensin standard sample, dissolve it in methanol and mix at a constant volume to make the monensin concentration 1mg / ml;

[0027] Preparation of salinomycin standard stock solution: Accurately weigh a certain amount of salinomycin standard sample, dissolve in methanol and mix at a constant volume so that the concentration of salinomycin is 1 mg / ml;

[0028] Preparation of salinomycin standard stock solution: Accurately weigh a certain amount of salinomycin standard sample, dissolve it in methanol and mix at a constant volume so that the concentration of salinomycin is 1 mg / ml;

[0029] Lasalocid sodium standard stock solution preparation: directly use the purchased standard with a concentration of 100 μg / ml, or other concentration standard reference substances can also be u...

Embodiment 2

[0047] Example 2 Verification, addition and recovery detection experiment

[0048] Feed samples without monensin, salinomycin, methyl salinomycin and lasalocid sodium were used as blank samples and crushed into powder. Take by weighing 2g sample in the 50ml centrifuge tube, add appropriate monensin, salinomycin, methyl salinomycin and lasalocid sodium standard solution respectively and make mixed feed sample, make four kinds of antibiotics in mixed feed The concentrations in the final filtrate of the samples were 10, 20, 60 μg / ml, respectively. Converted to the concentrations in the mixed feed samples were 5, 10, 30 mg / kg.

[0049] Add 10ml of 10% methanol solution by volume to the above mixed feed sample, shake it on a vortex mixer for 5 minutes, let it stand for 10 minutes, then centrifuge it at a speed of 5200r / min for 10 minutes, take the supernatant, and repeat the above extraction step once. The two supernatants were combined and dried with nitrogen to obtain a resid...

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Abstract

The invention relates to a method for detecting polyether antibiotics in feed. The method comprises the following steps of: passing through a mixed acidic mobile phase, and simultaneously separating lasalocid, monensin, salinomycin and narasin on a chromatographic separation column, namely extracting sample filtrate of the feed; removing air in the mixed acidic mobile phase by a degassing machine, then passing through a liquid phase pump, flowing into a high performance liquid chromatographic instrument via a safety switch, and adding the sample filtrate through a sample adding device; performing fluorescence detection on substances after separation by the chromatographic column to obtain a detection result of the lasalocid; introducing a mixed solution after detection into a reactor A through a backflow protector, and adding a derivative reagent A into the reactor A, wherein the reaction temperature is room temperature; then leading the mixed solution after reaction into a reactor B, and adding a derivative reagent B, wherein the reaction temperature is 90 DEG C; and detecting the solution after reaction so as to obtain the detection results of the monensin, the salinomycin and the narasin.

Description

technical field [0001] The invention belongs to the detection field of antibiotics, in particular to a method for quantitatively determining multiple polyether antibiotics at one time and at the same time. Background technique [0002] Lasalocid sodium, monensin, salinomycin and methyl salinomycin belong to polyether antibiotics and are ionophore antibiotics produced by actinomycete fermentation broth. It is currently the most widely used anticoccidiostat in poultry farming, and it is mainly added to feed to increase feed utilization and prevent coccidiosis. However, with the deepening of its application in animal disease prevention, the drug resistance of coccidia continues to increase, so the dosage is also increasing, resulting in an increase in residues in livestock and poultry products. Therefore, it is necessary to monitor the amount of polyether antibiotics added in the feed to ensure the health of the people. [0003] Currently, liquid chromatography is commonly us...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 陈明龚兰邵明诚魏瑞成
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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