Establishing method and application of fingerprint of detoxifying and kidney harmonizing capsule

A technology for detoxifying and calming the kidney and establishing a method, which is applied in the establishment of fingerprints of Qingdu and Anshen capsules and the establishment of fingerprints of traditional Chinese medicine compound preparations. Composition etc.

Active Publication Date: 2013-12-04
LI MIN PHARM FAB OF LIVZON PHARM GRP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the existing detection methods for the active ingredients of Qingdu Anshen Capsules are not perfect enough, and cannot fully, accurately, and effectively reflect various active ingredients...

Method used

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  • Establishing method and application of fingerprint of detoxifying and kidney harmonizing capsule
  • Establishing method and application of fingerprint of detoxifying and kidney harmonizing capsule
  • Establishing method and application of fingerprint of detoxifying and kidney harmonizing capsule

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Experimental program
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Embodiment 1

[0038] 1 Materials and Instruments

[0039] 1.1 Materials: Qingdu Anshen Capsules, provided by Limin Pharmaceutical Factory of Livzon Group. The reagents acetonitrile and formic acid used in the experiment were chromatographically pure, and the water was ultrapure water.

[0040] 1.2 Instrument: Agilent liquid mass spectrometer (1290UHPLC binary gradient pump, built-in vacuum degasser, 100-position autosampler, intelligent column thermostat, G6520B high-precision quadrupole tandem time-of-flight mass spectrometer system); chromatography Column: Agilent C 18 Reverse-phase bonded silica gel chromatographic column (Angilent Zorbax Eclipse Plus C18), specification: 2.1×100mm, packing particle size 1.8μm.

[0041] 2 Experimental methods and results

[0042] 2.1 Preparation of the test solution: take 10 capsules of Qingdu Anshen Capsules, remove the capsule shell, collect the contents, mix and finely grind the contents, accurately weigh 0.5g, place in a 100ml volumetric flask, ad...

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Abstract

The invention provides an establishing method of the fingerprint of a detoxifying and kidney harmonizing capsule, and an application of the fingerprint. The establishing method comprises the following steps: 1, dissolving the content of the detoxifying and kidney harmonizing capsule in an 70%(V) aqueous ethanol solution to prepare a sample solution having a concentration of 5mg/ml; and 2, detecting the sample solution by adopting a high performance liquid chromatograph-tandem mass spectrometry to obtain a total ionic chromatogram, wherein a high performance liquid chromatograph adopts a C18 reversed-phase bonded silica gel chromatography column, a mobile phase 1 and a mobile phase 2 are used for gradient elution, an electro-spray detector is used for detection, the mobile phase 1 is a 0.1%(V) aqueous solution of formic acid, and the mobile phase 2 is a 0.1%(V) formic acid-acetonitrile solution. The method has the advantages of high detection accuracy and reliability when the method is used for obtaining the fingerprint, and the obtained fingerprint can rapidly, simply, comprehensively and effectively display the effective components of the detoxifying and kidney harmonizing capsule and the contents of the effective components.

Description

technical field [0001] The invention belongs to the field of drug detection, and relates to a method for establishing a fingerprint of a compound traditional Chinese medicine preparation and its application, in particular to a method for establishing a fingerprint of Qingdu Anshen Capsule and its application. Background technique [0002] Traditional Chinese medicine compound preparations refer to preparations that combine two or more traditional Chinese medicines and apply them to the treatment of diseases. Because of its complex composition, it is very difficult to accurately detect its active ingredients. [0003] Traditional Chinese medicine fingerprint refers to the chromatogram or spectrum that can indicate the characteristics of the main chemical components of the traditional Chinese medicine, including Chinese medicinal materials, decoction pieces, extracts or Chinese patent medicines, which are obtained by certain analytical methods after appropriate processing. In...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 宋艳刚刘学华黄文华刘东来胡淑毅孙虹曹晖沈琳江晓漫
Owner LI MIN PHARM FAB OF LIVZON PHARM GRP
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