Alcaligenes faecalis strain

A technology for Alcaligenes faecalis and strains, applied in the directions of bacteria, fungicides, biocides, etc., can solve the problems of easy degradation of strains and unstable field control effect.

Inactive Publication Date: 2013-12-11
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the indoor inhibitory effect of the existing antagonistic bacteria is relatively ideal, the control effect i

Method used

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  • Alcaligenes faecalis strain
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  • Alcaligenes faecalis strain

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Embodiment 1

[0019] The preparation of embodiment 1 bacterial strain B137W of the present invention

[0020] Isolation of strains by dilution separation: soil samples were taken from the rice and duck co-culture field of the scientific research base of Hunan Agricultural University in Wulong Village, Liuyang City, Hunan Province, and 90 mL of sterile water was added to a triangular flask containing 10 g of soil samples, shaken for 30 minutes, and filtered with filter paper After standing still for 10min, the suspension was taken and diluted to 1×10 according to the gradient concentration method. -7 times, and then spread the diluted solution on the beef extract peptone agar medium plate, put it in a 28°C incubator and culture it continuously for 24 hours. strain.

[0021] Screening by plate confrontation method: make a suspension of the obtained single colony with sterile water, take a circular sterile filter paper piece with a diameter of 5 mm, and infiltrate enough suspension to make it...

Embodiment 2

[0023] Embodiment 2 The preparation of bacterial strain fermentation filtrate of the present invention

[0024] Pick 2-3 rings of the strain B137W of the present invention with an inoculation loop, insert them into 3000mL beef extract peptone liquid medium (volume 10000mL) and culture with continuous shaking at 28-30°C for 72 hours to obtain the fermentation broth of the strain B137W of the present invention. Take the fermentation liquid and filter it with filter paper first, and then filter it with a 0.22 μm bacterial filter to obtain the fermentation filtrate of bacterial strain B137W of the present invention.

Embodiment 3

[0025] Embodiment 3 The inhibitory action of bacterial strain of the present invention to rice sheath blight

[0026] Take the above-prepared fermentation filtrate and put it into a conical flask, evaporate and remove water in a water bath at 40°C, weigh the amount of the active substance, and prepare a solution with a concentration of 5 mg / mL with sterile water. Take 1 mL of the fermentation filtrate and 9 mL of potato Glucose agar medium was mixed and shaken to form a plate. Rice sheath blight bacteria cake with a diameter of 5 mm was inoculated and placed in the center of the plate. At the same time, Jinggangmycin (60% mass concentration of Jinggangmycin original medicine was dissolved in sterile water and prepared to 75 μg / mL) was used as the drug control. Water was used as a blank control, and each treatment was repeated 3 times, and cultured at 28-30°C. After 48 hours, the colony diameter was measured, and the inhibition rate was calculated. The results are shown in Tabl...

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Abstract

An Alcaligenes faecalis B137W is preserved in China Center for Type Culture Collection on May 7, 2013, and has a preservation number of CCTCC NO:M2013176. A fermentation filtrate of the above strain has an inhibition effect on Rhizoctonia solani, Fusarium graminearum, Colletotrichum capsici and Alternaria alternate, the inhibition effect of the fermentation filtrate on the Rhizoctonia solani is strongest, and the inhibition rate of the fermentation filtrate of the Alcaligenes faecalis B137W to the Rhizoctonia solani is 60.26% and is equal to the inhibition of validamycin to the Rhizoctonia solani, so the stain can be developed into bio-control agents.

Description

technical field [0001] The invention belongs to the field of biotechnology, and mainly relates to a strain of Alcaligenes faecalis which has a good inhibitory effect on rice sheath blight bacteria. Background technique [0002] Rice is an important food crop in my country, but it is disturbed by various factors during the growth process, among which rice sheath blight (Rhizoctonia solani) is one of the important factors affecting rice production, and the annual loss caused by it can reach 10- 30%. In recent years, with the promotion of high-yielding, short-stalked, multi-tiller varieties and the improvement of cultivation techniques such as direct seedling throwing, the occurrence of sheath blight has become increasingly serious. In terms of production, chemical pesticides were mainly used for prevention and control in the past, but Jinggangmycin is mainly used now because of problems such as pesticide residues, environmental pollution, and ecological balance damage. However...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
Inventor 张亚廖晓兰苏品黄璜刘双清蒋程
Owner HUNAN AGRICULTURAL UNIV
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