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Electric pressurized on-line enrichment capillary electrophoresis analysis method for aromatic carboxylic acids by coating inner walls of capillaries

A capillary electrophoresis, aromatic carboxylic acid technology, applied in the direction of material analysis by electromagnetic means, analysis of materials, preparation of samples for testing, etc.

Inactive Publication Date: 2013-12-25
DEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the prior art are that the enrichment factor of aromatic carboxylic acids in capillary electrophoresis analysis is low, and the precision of electroosmotic flow on the inner wall of capillary is low, which affect the accuracy of analytical data

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] Use tetrabutylamine hydroxide to dynamically coat the inner wall of the capillary (rinse with 1mol / L sodium hydroxide at 20psi for 20 minutes, rinse with water at 20psi for 20 minutes, rinse with 0.005mol / L tetrabutylamine hydroxide at 20psi for 20 minutes, rinse with water at 20psi for 20 minutes Minutes) to divert the electroosmotic flow of capillary electrophoresis; 2-nitrobenzoic acid and 3 -Separation of nitrobenzoic acid; before sample injection, enter the leading electrolyte under 0.5psi pressure for 20s, then introduce the sample at -10kV for 100s, and then use 3-(cyclohexylamino)-1-propane sulphonic acid as the final electrolyte to achieve The online enrichment and separation of 2-nitrobenzoic acid and 3-nitrobenzoic acid by electro-supercharging achieved enrichment factors of 2826 and 3106 times respectively. The precision is not more than 3.5%.

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Abstract

The invention discloses an electric pressurized on-line enrichment capillary electrophoresis analysis method for aromatic carboxylic acids by coating inner walls of capillaries. The electric pressurized on-line enrichment capillary electrophoresis analysis method comprises following steps: firstly, capillary inner wall dynamic coating is realized by using tetrabutylammonium hydroxide, and the direction of electroosmotic flow is turned around; in a 30mmol / L borax buffer solution containing 8mmol / L beta-CD, separation of 2-nitrobenzoic acid and 3-nitrobenzoic acid under homodromous electroosmotic flow conditions is realized, wherein the pH value of the 30mmol / L borax buffer solution is 9.0; after 20s of addition of a lead electrolyte at a pressure of 0.5psi, and 100s of addition of a sample under a pressure of -10kV, electric pressurized on-line enrichment and separation of 2-nitrobenzoic acid and 3-nitrobenzoic acid are realized by taking 3-(cyclohexylamino)-1-propane sulphonic acid as a final electrolyte.

Description

technical field [0001] The invention relates to the field of capillary electrophoresis analysis, in particular to a method for capillary inner wall coating with electric boost and online enrichment capillary electrophoresis analysis method for aromatic carboxylic acids. Background technique [0002] Capillary electrophoresis is the product of the combination of classical electrophoresis technology and modern microcolumn separation. It is a new electrophoresis technology that uses electric field as the driving force to efficiently and rapidly separate ions or charged particles in capillaries according to their mobility or / and distribution coefficient. In the widely used zone capillary electrophoresis (capillary zone electrophoresis, CZE), the capillary and the electrode tank are filled with the background electrolyte solution (buffer solution) of the same composition and concentration. The sample is introduced from one end of the capillary (called the injection end). When a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N1/40
Inventor 吕元琦
Owner DEZHOU UNIV