Kit used for detecting sheeppox virus

Abstract

The invention discloses a kit used for detecting sheeppox virus. The kit comprises at least four primers which are represented by SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4 respectively; dNTP, Tris-HC, KCl, (NH4)2SO4, MgSO4, Tween20, Bst enzyme and template DNA. The kit processes advantages such as high specificity, relatively simple detect operation, and fast detection speed; and can be used in capripox virus epidemiologic studies and disease control and prevention.

Description

technical field [0001] The invention relates to a detection kit, specifically a kit for detecting sheep pox virus. Background technique [0002] Capripox (CP) includes sheep pox, goat pox and bovine skin pimple disease, the first two of which are caused by sheep pox virus (Sheeppox virus, SPPV) and goat pox virus (Goatpox virus, GTPV) respectively. contact infectious diseases. The body temperature of sick animals rises, papules or nodules, blisters, visceral lesions and even death appear on the whole body, which has caused serious economic losses to the sheep industry in the world. The World Organization for Animal Health (OIE) stipulates it as an animal disease that must be reported by law, and my country lists it as a first-class animal infectious disease. [0003] The differential diagnosis of sheep pox and goat pox has been a research hotspot. Studies have shown that the homology of SPPV and GTPV gene sequences reaches 96%-97%, and there are only certain degree of seq...

AI Technical Summary

Problems solved by technology

It can be seen that the above-mentioned published methods or kits cannot distinguish viruses of the same genus and different species, that is to say, they cannot distinguish the genus of goat pox virus from the book of goat pox virus

In addition, the detection sensitivity of these detection kits that have been disclosed is also low

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