PTEN (phosphatase and tensin homolog) gene interference shRNA3, and recombinant adenovirus vector and construction thereof

A technology of recombinant adenovirus and gene interference, which is applied in the field of animal molecular biology and genetic engineering, can solve the problems of gene mutation and carcinogenesis, and achieve the effect of promoting the accumulation of fat
CN103497952BActive Publication Date: 2014-12-10HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Publication Date
2014-12-10

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Abstract

The invention relates to a PTEN (phosphatase and tensin homolog) gene interference shRNA3 of which the nucleotide sequence is shown in SEQ ID No.1. The invention also provides a recombinant adenovirus vector containing the PTEN gene interference shRNA3 as well as a construction method and application thereof. According to the invention, by selecting an RNAi (RNA interfere) technology, a pAdxsi-GFP-PTEN-shRNA vector is successfully constructed, and through culturing and amplifying, an interference recombinant adenovirus with a titer of 1.2*10<10> PFU (Plaque Forming Unit) / ml is obtained, so that the expression of PTEN genes can be reduced, and due to the low expression of PTEN, the assembling and secretion of VLDL (very low-density lipoprotein) are reduced, and then the accumulation of intracellular TG is caused, thereby promoting accumulation of fats in liver cells.
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Description

technical field

[0001] The invention belongs to the field of animal molecular biology and genetic engineering, and relates to a PTEN gene interference shRNA3 and a recombinant adenovirus vector and its construction. Background technique

[0002] Fatty liver of dairy cows during the peripartum period is a metabolic disease caused by the negative energy balance of dairy cows during the transition period. However, due to the lack of effective control measures, it has brought huge losses to the cattle industry, and it is urgent to find an effective treatment method. In view of the weak liver fatty acid oxidation ability of peripartum dairy cows (especially Holstein cows), the insufficient ability to synthesize and secrete lipoproteins is the main link in the occurrence of fatty liver, and the establishment of gene therapy to regulate liver fatty acid oxidation and VLDL secretion and synthesis may be possible. It can provide new ideas for the prevention and treatment of fatty liv...

Claims

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