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Method for identifying and screening dendritic killer cells

A technology of dendritic cells and identification methods, applied in animal cells, vertebrate cells, biochemical equipment and methods, etc., can solve the problems of cumbersome and limited interferon-secreting killer dendritic cells, etc.

Active Publication Date: 2014-01-22
ACAD SINIC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as mentioned above, although interferon-secreting killer dendritic cells play a very important role in the immune response, because the amount of interferon-secreting killer dendritic cells in the body is very rare, such a low cell ratio However, the number of interferon-secreting killer dendritic cells requires cumbersome steps to purify, which also limits further research on interferon-secreting killer dendritic cells. Not only that, the current technology can only isolate interferon-secreting killer dendritic cells from the spleen, lymph nodes or bone marrow of mice. Killer dendritic cells have faced considerable bottlenecks in their practical application in research or clinical practice

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  • Method for identifying and screening dendritic killer cells
  • Method for identifying and screening dendritic killer cells
  • Method for identifying and screening dendritic killer cells

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Embodiment

[0044] Please refer to figure 1 and Figure 2A Figure to Figure 2D . figure 1 A flowchart showing a screening method for dendritic killer cells according to an embodiment of the present invention, Figure 2A Figure to Figure 2D It shows the results of sorting dendritic killer cells in the peripheral blood of cancer patients by flow cytometry in an embodiment of the present invention.

[0045] First, in step S100, 40 ml of whole blood was collected from healthy people or cancer patients, diluted with the same volume of phosphate buffered saline (PBS), and separated from human peripheral blood mononuclear cells ( Peripheral blood mononuclear cell, PBMC). Basically, human peripheral blood mononuclear cells can be divided into five types of cells, such as: Monocytes (Monocytic cells), small cells (small cells), lymphocytes (Lymphoid cells), large cells (large cells) and large granule cells (large and granular cells). Therefore, the present invention utilizes a flow cyto...

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Abstract

A method for identifying and screening dendritic killer cells (DKC) is disclosed in the present invention, and the method is to identify a plurality of cell surface markers selected from a group consisting of HLA-G-, CD14-, CD3-, CD19-, CD56dim and HLA-DR+. The method for screening DKC from human peripheral blood mononuclear cells (PBMC) comprises the following steps. A first cell population with regular cell size is provided and a second cell population is then sorted out from the first cell population by screening the phenotype of CD14 and HLA-G therein. A third cell group is further sorted out from the second cell population by screening the phenotype of CD19 and CD3 therein. Finally, DKC will be identified out of the third cell population by screening the phenotype of CD56 and HLA-DR.

Description

technical field [0001] The invention relates to a method for identifying and screening dendritic killer cells, which mainly uses the characteristics of different surface markers of different groups of cells to sort the cell groups, and finally screens the dendritic killer cells from human peripheral blood mononuclear cells. HLA - G - CD14 - CD19 - CD3 - CD56 dim HLA-DR + approach to dendritic killer cells. Background technique [0002] The human body has a defense mechanism to identify foreign objects and initiate a series of reactions. This defense system is the immune system. The immune system has cells with different functions, such as white blood cells and lymphocytes, and protein factors with different functions, such as immunoglobulins, interleukins, and cytokines, which coordinate and cooperate with each other to form the body's defense force. The immune response is divided into congenital and acquired according to its specificity and memory. The innate immun...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N5/078
CPCG01N33/5094G01N33/56972G01N33/56977
Inventor 李建谋
Owner ACAD SINIC