Polyploid breeding method for eucalyptus camaldulensis 201-2

A technology of polyploid breeding and red eucalyptus, which is applied in the field of plant tissue culture, can solve problems such as hindering the healthy development of eucalyptus, and achieve the effect of strong induction, good effect and reduced toxicity

Inactive Publication Date: 2014-02-05
CHONGQING UNIV OF ARTS & SCI
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Problems solved by technology

At present, the forestry industry has an increasing demand for industrial raw material forests with a s

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  • Polyploid breeding method for eucalyptus camaldulensis 201-2
  • Polyploid breeding method for eucalyptus camaldulensis 201-2
  • Polyploid breeding method for eucalyptus camaldulensis 201-2

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Embodiment Construction

[0029] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0030] The test material Eucalyptus urocarpa 201-2 aseptic tissue culture seedlings used in the examples of the present invention was provided by the Flower Research Engineering Center of Chongqing University of Arts and Sciences.

[0031] 1. Polyploid induction of Eucalyptus urocarpa 201-2

[0032] Take 4-6 leaves from the upper part of the plant, remove 1 / 3 of the leaf tips, cut scars on the leaves, and keep the petioles. Soak 10 treated leaves in colchicine reagent bottles with different concentrations, the colchicine concentration is set to three gradients of 0.1%, 0.2%, and 0.3%; the time of treatment is 12-72h; each group of materials Repeat 3 times; during induction, oscillate in a shaker at a speed of 120r / min. The entire colchicine-induced doubling process was performed under sterile conditions to ensure that the tissue-cultured ...

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Abstract

The invention discloses a polyploid breeding method for eucalyptus camaldulensis 201-2. The polyploid breeding method comprises the following steps: (1) placing leaves of a tissue culture seedling of the eucalyptus camaldulensis 201-2 into colchicine with the mass concentration being 0.2% and immersing for 24 hours; and (2) inoculating the leaves which are immersed by the colchicine in the step (1) into a proliferation culture medium for culturing; and carrying out subculture on differentiated adventitious buds and inoculating the adventitious buds to a rooting culture medium for culturing to obtain eucalyptus camaldulensis 201-2 polyploid plants. According to the polyploid breeding method for the eucalyptus camaldulensis 201-2, a colchicine immersing method is used for inducing and treating to carry out polyploid induction and a tissue culture technology is combined to culture; a suitable induction scheme is obtained by analyzing through different concentrations and different processing times of the colchicine on the polyploid induction, and the eucalyptus camaldulensis 201-2 polyploid plants are cultured. The method disclosed by the invention not only has the characteristics of economy, convenience, strong specificity on an induction effect, reduction of the toxicity and the like, but can also improves the generation frequency of polyploids and reduces the appearance of chimeras.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a polyploid breeding method of Eucalyptus urocarpa 201-2. Background technique [0002] Eucalyptus is the collective name for plants of the genus Eucalyptus of Myrtaceae (Myrtaceae). It is an evergreen tall tree (a few species are small trees). Features such as wide space. Eucalyptus tree is beautiful, evergreen in all seasons, grows rapidly, has the ability to germinate and renew and improve swamps, and is suitable for landscaping tree species; the leaves contain aromatic oil, which has the effect of sterilizing and repelling mosquitoes, and can be extracted from sesame oil; twigs and bark contain Tannins can be extracted from tannins; bark and wood can also be used to make pulp, construction, furniture, etc. Eucalyptus likes warm climates, but it is not resistant to heat and humidity. It grows poorly in overheated climates, is afraid of cold, and is particularly se...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈泽雄刘奕清
Owner CHONGQING UNIV OF ARTS & SCI
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