Polyploid breeding method for eucalyptus camaldulensis 201-2
A technology of polyploid breeding and red eucalyptus, which is applied in the field of plant tissue culture, can solve problems such as hindering the healthy development of eucalyptus, and achieve the effect of strong induction, good effect and reduced toxicity
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[0029] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
[0030] The test material Eucalyptus urocarpa 201-2 aseptic tissue culture seedlings used in the examples of the present invention was provided by the Flower Research Engineering Center of Chongqing University of Arts and Sciences.
[0031] 1. Polyploid induction of Eucalyptus urocarpa 201-2
[0032] Take 4-6 leaves from the upper part of the plant, remove 1 / 3 of the leaf tips, cut scars on the leaves, and keep the petioles. Soak 10 treated leaves in colchicine reagent bottles with different concentrations, the colchicine concentration is set to three gradients of 0.1%, 0.2%, and 0.3%; the time of treatment is 12-72h; each group of materials Repeat 3 times; during induction, oscillate in a shaker at a speed of 120r / min. The entire colchicine-induced doubling process was performed under sterile conditions to ensure that the tissue-cultured ...
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