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Polysaccharide/PEI nanogel with reduction responsiveness, preparation, and preparation method of polysaccharide/PEI nanogel

A nano-gel and polysaccharide technology, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, antibody medical ingredients, etc. It can solve the problems of large particle size, insensitivity to the difference between the internal and external environments of cells, and the inability to load hydrophobic substances. , to achieve the effect of accelerating the release speed

Active Publication Date: 2014-02-19
珠海中科先进科技产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first prior art and the second prior art are mainly nanoparticles formed based on the electrostatic interaction of positive and negative charges, which have a large particle size and cannot carry hydrophobic substances; insensitive to the difference

Method used

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  • Polysaccharide/PEI nanogel with reduction responsiveness, preparation, and preparation method of polysaccharide/PEI nanogel
  • Polysaccharide/PEI nanogel with reduction responsiveness, preparation, and preparation method of polysaccharide/PEI nanogel
  • Polysaccharide/PEI nanogel with reduction responsiveness, preparation, and preparation method of polysaccharide/PEI nanogel

Examples

Experimental program
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Effect test

preparation example Construction

[0051] The preparation method of the reduction-responsive polysaccharide PEI nanogel in the present invention comprises the following steps:

[0052] (1) The electrostatic interaction between sodium alginate and PEI forms a gel

[0053] Adding the sodium alginate solution into the PEI solution, stirring at the same time, placing it at room temperature for reaction to obtain a first reaction solution; filtering the first reaction solution and performing dialysis to obtain a first nanogel;

[0054] (2) Disulfide cross-linking

[0055] Mix and stir the first nanogel obtained in step (1) with the disulfide crosslinking agent, react at room temperature to obtain a second reaction solution; filter the second reaction solution and dialyze to obtain a reduction-responsive polysaccharide PEI nanogel glue.

[0056] The PEI used in the examples of the present invention is branched and has a molecular weight of 2000.

[0057] The disulfide crosslinking agent of the present invention is s...

Embodiment 1

[0059] Reducing polysaccharide PEI nanogel:

[0060] Solution configuration:

[0061] Preparation of PEI solution: PEI was dissolved in 2-(N-morpholino)ethanesulfonic acid buffer solution (MES buffer solution)-so that the final concentration of PEI was 1mg / ml;

[0062] Preparation of sodium alginate solution: Sodium alginate was dissolved in 2-(N-morpholino)ethanesulfonic acid buffer solution (MES buffer solution) so that the final concentration of sodium alginate was 1 mg / ml.

[0063] Preparation of reduction-responsive polysaccharide PEI nanogels, the steps are as follows:

[0064] 1. Electrostatic interaction between sodium alginate and PEI to form a colloid: put the prepared PEI solution in a round-bottomed flask, then slowly drop the sodium alginate solution with the same volume as the added PEI solution into the PEI solution and mix it with magnetic stirring at the same time Stir with a device, and react at room temperature for 24 hours; filter the reacted solution wit...

Embodiment 2

[0088] For the reducing polysaccharide PEI nanogel, the amount of disulfide crosslinking agent used is different from that of Example 1.

[0089] Solution configuration:

[0090] Preparation of PEI solution: PEI was dissolved in 2-(N-morpholino)ethanesulfonic acid buffer solution (MES buffer solution)-so that the final concentration of PEI was 1mg / ml;

[0091] Preparation of sodium alginate solution: Sodium alginate was dissolved in 2-(N-morpholino)ethanesulfonic acid buffer solution (MES buffer solution) so that the final concentration of sodium alginate was 1 mg / ml.

[0092] Preparation of reduction-responsive polysaccharide PEI nanogels, the steps are as follows:

[0093] 1. Electrostatic interaction between sodium alginate and PEI to form a colloid: put the prepared PEI solution in a round-bottomed flask, then slowly drop the sodium alginate solution with the same volume as the added PEI solution into the PEI solution and mix it with magnetic stirring at the same time St...

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Abstract

The invention discloses a polysaccharide / PEI nanogel with reduction responsiveness, and a preparation method of the polysaccharide / PEI nanogel. The preparation method comprises following steps: a gel is prepared from a polysaccharide and PEI via electrostatic interaction; and then a disulfide cross-linking agent is added for crosslinking, and the polysaccharide / PEI nanogel with reduction responsiveness is obtained. The polysaccharide / PEI nanogel processes following ingredients, by weight, 1 to 100 parts of the polysaccharide, 1 to 100 parts of PEI, and 10 to 2000 parts of the disulfide cross-linking agent. The polysaccharide / PEI nanogel possesses low toxicity; is capable of encapsulating genes or proteins preferably, and protecting substances encapsulated by the polysaccharide / PEI nanogel from degradation effect of lysozyme, and from cytophagy by immune cells; is capable of realizing response on reducing conditions in cells, and increasing release rate of the substances encapsulated by the polysaccharide / PEI nanogel; and can be used as a nanogel carrier to load proteins, polypeptides or nucleic acids. The polysaccharide / PEI nanogel also possesses effects of an immunologic adjuvant. All processes of the preparation method are performed in water, and no environmental pollution is caused.

Description

technical field [0001] The invention relates to a novel nanogel, in particular to a reduction responsive nanogel. Background technique [0002] Nanocarriers refer to nanoscale systems that can carry target substances such as genes or proteins. Nanocarriers wrap molecules such as DNA, RNA, or protein inside or adsorbed on the surface of nanoparticles, and introduce target substances into cells under the action of cell uptake, so as to achieve safe and effective treatment or other effects. Nanocarriers have certain advantages in mediating gene or protein transfer. First, nanomaterials are generally biocompatible and biodegradable, so they are basically non-toxic and immunogenic, and will not cause the body’s immune response or cause Cell transformation and death. Moreover, nanoparticles have special structures and surface charges, and have high transfer efficiency of genes or proteins. Nanocarriers can also mediate the integration of exogenous genes in the chromosomal DNA o...

Claims

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Application Information

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IPC IPC(8): C08L5/04C08L79/02C08K5/42C08K5/36C08K5/372C08K5/37C08J3/24C08J3/075A61K39/39A61K39/00
Inventor 马轶凡李萍刘朋蔡林涛
Owner 珠海中科先进科技产业有限公司
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