Method for identifying infection condition of gall disease of cryptomeria fortunei

A technology for cedar and gall tumor, which is applied in the field of identifying the infection status of cedar gall disease, can solve the problems that have not yet occurred, and achieves the effects of non-destructive detection process, convenient operation, high safety and repeatability

Inactive Publication Date: 2014-03-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] It can be seen that FT-IR technology can be used for accurate and rapid detection and identification of microorganisms, but there are no related reports on the application of FT-IR to the detection of gall tumor pathogens

Method used

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  • Method for identifying infection condition of gall disease of cryptomeria fortunei
  • Method for identifying infection condition of gall disease of cryptomeria fortunei
  • Method for identifying infection condition of gall disease of cryptomeria fortunei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Acquisition of characteristic peaks of cedars infected with galls and uninfected cedars

[0035] 1 Preparation of pellets for infrared analysis

[0036] 1) Sample collection: Branches and needles of Cryptomeria cedar infected with gall tumor and branches and needles of Cryptomeria uninfected with gall tumor were collected in Tianmu Mountain, Zhejiang Province;

[0037] 2) Freeze-drying and grinding: freeze-dry the collected cedar cedar samples at -70°C, and then grind them into sample powders with a particle size of less than 2 μm in a mortar;

[0038] 3) Potassium bromide preparation: put the potassium bromide (KBr) powder in a desktop oven, bake at 120°C for 24 hours for drying treatment, grind the potassium bromide powder in a clean agate mortar and pass it for 200 Mesh sieve, dry under infrared light for 4h, put into desiccator and save for later use;

[0039] 4) Preparation of compressed tablets: Mix 100mg of dried potassium bromide with 1mg of sample p...

Embodiment 2

[0063] Example 2 Identification of gall tumor infection status of cedar to be tested

[0064] Take cedar branch sample D1 known to be infected with gall disease, and cedar branch sample W1, branch sample S1, and needle sample S2 of cedar branch sample S2 with unknown gall disease infection status; The disease infection status of cedar was identified.

[0065] 1 Morphological observation of pathogenic bacteria

[0066] Cut tissue pieces from D1, W1, S1, and S2 samples respectively, disinfect them with sodium hypochlorite for 7 to 8 minutes, rinse them with sterile water for 4 to 5 times, and put the tissue pieces into potato dextrose agar (PDA) containing cedar juice for culture. cultured at a constant temperature of 25°C, and purified after the formation of colonies

[0067] Cultivate, record the characteristics of the colonies and perform microscopic examination. The observation results are shown in Table 3.

[0068] Table 3 Pathogen Morphological Observation Results of Each...

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Abstract

The invention discloses a method for identifying the infection condition of the gall disease of cryptomeria fortunei. The method comprises the following steps: drying a proper amount of branches or needles of cryptomeria fortunei to be detected, grinding into powder, mixing the powder and potassium bromide, and tableting the mixture; collecting an infra-red spectrogram of the tablet; determining whether the cryptomeria fortunei to be detected is infected with gall disease or not according to the infrared spectrogram. According to the method, the difference between characteristic peaks of infrared spectrograms of healthy cryptomeria fortunei and cryptomeria fortunei infected with gall disease can be discovered by utilizing the Fourier transform infrared spectroscopy technology, and whether the cryptomeria fortunei is infected with pathogen of the gall disease or not can be detected by utilizing the difference, the detection process is non-destructive, quick, accurate and convenient to operate, is independent of human experience, has high safety and repeatability, is a new technical means provided to effectively prevent and control the gall disease of cryptomeria fortunei.

Description

technical field [0001] The invention belongs to the technical field of detection of plant pathogenic bacteria, and in particular relates to a method for identifying the infection status of cedar gall disease. Background technique [0002] Cryptomeria fortunei is an evergreen coniferous tree belonging to the Taxodiaceae genus Cryptomeria. It is a national first-class protected tree species and is mainly distributed in the south of the Yangtze River. Cryptosporiopsis fungi can cause cedar galls, which mainly occur on the trunk, branches and twigs of cedar, which can lead to leaf fall, branch wilting, and even the death of the whole cedar. Gall tumor pathogens are mainly transmitted by airflow and rainwater, and the wounds caused by acid rain corrosion invade the branches or trunks of cedar. [0003] In recent years, goiter disease has spread continuously in the cedar population, becoming a very serious disease that causes the whole plant to decline, seriously reducing the orn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563
Inventor 李斌王丽葛梦禹单长林陈晓玲谢关林
Owner ZHEJIANG UNIV
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