Application of Rana extract in preparation of medicine for treating and preventing cervical cancer

A technology for extracting liquid and cervical cancer, which is applied in the directions of drug combination, anti-tumor drugs, liquid delivery, etc., to achieve the effects of rapid disappearance of clinical symptoms, less toxic and side effects, and less resistance to drug resistance.

Active Publication Date: 2016-05-18
孟繁清
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no application of Rana extract in medicines for the treatment and prevention of cervical cancer

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  • Application of Rana extract in preparation of medicine for treating and preventing cervical cancer
  • Application of Rana extract in preparation of medicine for treating and preventing cervical cancer
  • Application of Rana extract in preparation of medicine for treating and preventing cervical cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of Rana Rana Extract

[0021] Take 15g of dried wood frog (Chinese Rana subspecies Changbai Mountain, purchased from Jilin Pharmacy) and grind it, add 20 times the volume (ml:g) of 9% acetic acid 300ml; ultrasonic extraction, ultrasonic time is 10min, ultrasonic temperature is 18℃ After ultrasonication, filter and centrifuge, the centrifugation temperature is 4°C, the centrifugation time is 16min, and the centrifugation speed is 5500rpm, to obtain the Rana extract.

Embodiment 2

[0022] Example 2 Preparation of Rana Rana Extract Ointment

[0023] Prescription composition (by weight): 24 parts of dried wood frog, 17 parts of paraffin, 45 parts of liquid paraffin, 18 parts of white petrolatum, 34 parts of glyceryl monostearate, 4029 parts of span, 6 parts of emulsifier OP, p-hydroxybenzene 3 parts of ethyl formate, 7 parts of borneol.

[0024] Preparation:

[0025] (1) Preparation of Rana extract: Weigh 20g of dried Rana (Chinese Rana Changbaishan subspecies, purchased from Jilin Pharmacy) and pulverize it, add 25 times the volume (ml:g) of 7% acetic acid and ultrasonically 500ml; ultrasonically Extraction, ultrasonic time is 60min, ultrasonic temperature is 4°C; after ultrasonication, filter and centrifuge, the centrifugation temperature is 4°C, the centrifugation time is 5min, the centrifugation speed is 4000rpm, and the Rana extract is obtained;

[0026] (2) Preparation of the oil phase: Grind and mix paraffin, glycerol monostearate, white petrola...

Embodiment 3

[0028] Example 3 Preparation of Rana Rana Extract Ointment

[0029] Prescription composition (by weight): 36 parts of dried wood frog, 14 parts of paraffin, 39 parts of liquid paraffin, 22 parts of white petrolatum, 37 parts of glyceryl monostearate, 4029 parts of span, 3 parts of emulsifier OP, p-hydroxybenzene 4 parts of ethyl formate, 8 parts of borneol.

[0030] Preparation:

[0031] (1) Preparation of Rana extract: Take 26g of dried Rana (Chinese Rana Changbaishan subspecies, purchased from Jilin Pharmacy) and grind it, add 15 times the volume (ml:g) of 6% acetic acid 390ml; ultrasonically extract, The ultrasonic time was 16 minutes, the ultrasonic temperature was 28°C; after ultrasonication, it was filtered and centrifuged, the centrifugation temperature was 28°C, the centrifugation time was 40 minutes, and the centrifugation speed was 8000rpm to obtain the Rana extract;

[0032] (2) Preparation of the oil phase: Grind and mix paraffin, glycerol monostearate, white p...

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Abstract

The present invention relates to the application of Rana extract in the preparation of medicines for the treatment and prevention of cervical cancer, and provides a new medical application of Rana extract, which utilizes the inhibitory and anti-inflammatory and antibacterial effects of Rana extract on SiHa cells and Caski Cervical cancer caused by cells can be treated and prevented; the present invention can also change the permeability of bacterial cell membranes, and it is not easy to produce drug resistance; pure biological preparations are selected, with small toxic and side effects, treating both symptoms and root causes, significant curative effect, and rapid disappearance of clinical symptoms. It also has a preventive effect.

Description

technical field [0001] The invention relates to the application of the extract of wood frog in the preparation of medicine for treating and preventing cervical cancer, and an ointment of the extract of wood frog for treating and preventing cervical cancer, belonging to the technical field of medical biopharmaceuticals. Background technique [0002] Cervical cancer is one of the most common gynecological malignancies, and its incidence rate ranks second among female malignancies, second only to breast cancer. About 470,000 new cases of cervical cancer occur each year, accounting for 5% of all new cancer cases, and 230,000 people die from the disease, 80% of which occur in developing countries. Current studies have confirmed that high-risk human papillomavirus (humanpapillomavirus, HPV) is the most important risk factor for cervical cancer, and its presence can be detected in 99.7% of invasive cervical cancer. More than 200 types of HPV have been identified, of which about 30...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/65A61K9/06A61P35/00
Inventor 孟繁清
Owner 孟繁清
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