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Fructan extract, and preparation method and application thereof

An extract and fructan technology, applied to the preparation field of the fructan extract, can solve the problems of high cost, long time consumption, inability to adapt to industrial production and the like, and achieve short time consumption, low cost, and reduced animal fat. The effect of content

Inactive Publication Date: 2014-03-26
SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current preparation methods for preparing fructan from Ophiopogon japonicus have defects such as time-consuming, high cost, and inability to adapt to industrial production, and cannot be mass-prepared
At the same time, in the prior art, there are few reports on the activity of fructan extracts in specific molecular weight ranges

Method used

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  • Fructan extract, and preparation method and application thereof
  • Fructan extract, and preparation method and application thereof
  • Fructan extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 (preparation of fructan extract)

[0027] Take 20kg of dried Ophiopogon japonicus (Zhejiang Ophiopogon japonicus) root tubers, extract twice with 3 times the weight of 90% ethanol under reflux, and discard the extract; The liquid was concentrated under reduced pressure to 1.0g / ml; the concentrated water extract solution was precipitated by adding 2 times the volume of 95% ethanol, left to stand, and centrifuged to remove the precipitate; Precipitate and dry to obtain fructan extract with molecular weight in the range of 2000-10000. Get fructan extract 3.06kg, yield is: 15.3%. The structure was identified as fructan.

Embodiment 2

[0028] Embodiment 2 (preparation of fructan extract)

[0029] Take 20kg of dried Ophiopogon japonicus (Zhejiang Ophiopogon japonicus) root tubers, reflux extract with 10 times the weight of 70% ethanol for 3 times, and discard the extract; The solution was concentrated under reduced pressure to 0.8g / ml; the concentrated solution was added to 3 times the volume of 70% ethanol for precipitation, left to stand, and centrifuged to remove the precipitate; the supernatant was then added to 6 times the volume of 85% ethanol to precipitate, and the resulting Precipitate and dry to obtain fructan extract with molecular weight in the range of 2000-10000. 2.13kg of fructan extract was obtained, and the yield was: 10.6%. The structure was identified as fructan.

Embodiment 3

[0030] Embodiment 3 (preparation of fructan extract)

[0031] Take 20kg of dried Ophiopogon japonicus (Zhejiang Ophiopogon japonicus) root tubers, extract once with 2 times the weight of 100% ethanol under reflux, and discard the extract; The liquid was concentrated under reduced pressure to 0.5g / ml; the concentrated solution of water extraction was precipitated by adding 1 volume of 90% ethanol, left to stand, and centrifuged to remove the precipitate; Precipitate and dry to obtain fructan extract with molecular weight in the range of 2000-10000. Get 2.01kg of fructan extract, yield: 10.0%. The structure was identified as fructan.

[0032] Known from embodiment 1-3, the yield of the fructan extract of embodiment 1 is the highest, therefore embodiment 1 is a preferred embodiment.

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Abstract

The invention discloses a fructan extract and a preparation method thereof. The preparation method comprises the following steps: extracting Zhejiang ophiopogon root tuber with ethanol to remove impurities, extracting with water, carrying out ethanol fractional precipitation, and drying to obtain a fructan extract (the molecular weight is 2000-10000). The method for preparing the fructan extract has the advantages of short time consumption and low cost, and can perform abundant preparation and adapt to industrial production. Besides, the invention also discloses application of the fructan extract in preparing medicines, foods or health products for losing weight. The inspection on functions of the fructan extract by an abdomen-fat mouse model proves that the fructan extract has an obvious action of losing weight.

Description

technical field [0001] The invention relates to a fructan extract, and also relates to a preparation method and application of the fructan extract. Background technique [0002] Fructans are a class of carbohydrates that are beneficial to human health. They are composed of multiple glucose molecules linked by β-sugar sugar bonds. Since the human body does not contain enzymes that can decompose fructan, it cannot digest and absorb fructan, but fructan can become a nutrient for intestinal bacteria after entering the colon. Human consumption of food containing fructan can selectively promote the growth of bifidobacteria and reduce the production of substances related to tumorigenesis. In addition, short-chain fructan can be used as a low-calorie sweet ingredient. Therefore, fructans are a class of soluble carbohydrates that are beneficial to human health. [0003] Obesity is a nutritional metabolic disorder disease caused by the joint action of genetic factors and environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/733A61P3/04A23L1/09A23L33/125
Inventor 冯怡阮克锋王源从文娟林晓
Owner SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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