Strain capable of controlling ulcer disease of actinidia and preparation method thereof

A strain and No. 1 technology are applied in the field of plant endogenous Streptomyces cyanogenus and the preparation of bacterial agents, which can solve the problems of drug resistance of cankers and affect the field control effect of cankers, and achieve low cost, stable product quality, and easy operation. convenient effect

Inactive Publication Date: 2014-03-26
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of copper preparations has caused the resistance of canker bacteria, and the food safety problems caused by the extensive use of antibiotics have seriously affected the field control effect of canker

Method used

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  • Strain capable of controlling ulcer disease of actinidia and preparation method thereof
  • Strain capable of controlling ulcer disease of actinidia and preparation method thereof
  • Strain capable of controlling ulcer disease of actinidia and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In vitro antibacterial activity test of TIASA5 bacterial strain bacterial agent of the present invention.

Embodiment 2

[0036] Field control test of TIASA5 strain inoculum against kiwifruit bacterial canker.

[0037] At the kiwi fruit experiment station of Northwest Agriculture and Forestry University in Meixian County, Shaanxi Province, the 5-6-year-old "Hayward" variety was selected as the control object.

[0038] On November 5, 2011, the 5-fold dilution of the TIASA5 bacterial strain bacterial agent and the 72% agricultural streptomycin diluted 1000 times and the 46.1% copper hydroxide diluted 1000 times were applied with clear water on November 5, 2011. as comparison. Brush 2 times in a row with an interval of 10 days. The experiment consisted of 12 lesions per treatment, with 3 repetitions. On April 20, 2012 (the peak period of local kiwifruit canker disease), the incidence was investigated, and the diseased plant rate and control effect were calculated.

[0039] Diseased plant rate (%) = diseased plant tree / total number of surveyed plants × 100%

[0040] Control effect (%) = (control ...

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Abstract

The invention relates to a strain capable of controlling ulcer disease of actinidia and a preparation method thereof, belongs to the technical field of biological pesticide and discloses a Streptomyces glaucus sp.nov.yangling TIASA5 strain. The strain was collected in China General Microbiological Culture Collection Center on August 16, 2007; and the collection number is CGMCC No.2132. The invention also discloses a separation and verification method of the strain and a preparation method of a microbial inoculant. The invention also discloses application of the TIASA5 strain microbial inoculant in controlling bacterial ulcer disease of actinidia. The TIASA5 strain is stable in natural sources, simple in screening method, simple to prepare, convenient to operate, stable in product quality, green and environment-friendly, and has remarkable control effect on bacterial ulcer disease of actinidia.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a preparation method and application of a new plant endogenous Streptomyces cyanogenus and bacterial agent. Background technique [0002] Kiwifruit bacterial canker (Kiwifruit bacterial canker) caused by Pseudomonas syringae pv. Restricting the foreign trade export of kiwifruit pollen and fruit in my country has become a major problem that needs to be solved as soon as possible in the current kiwifruit production. At present, streptomycin and copper preparations are mainly used for the prevention and treatment of kiwifruit bacterial canker. However, the extensive use of copper preparations has caused the drug resistance of canker bacteria, and the food safety problems caused by the extensive use of antibiotics have seriously affected the field control effect of canker. Therefore, finding and developing new and efficient biological pesticides will bring good new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/465
Inventor 黄丽丽高小宁康振生涂璇
Owner NORTHWEST A & F UNIV
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